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Cytokine secretion pattern in treatment of lymphocytes of multiple sclerosis patients with fumaric acid esters.
Cytokine secretion pattern in treatment of lymphocytes of multiple sclerosis patients with fumaric acid esters.
- Source :
-
Immunological investigations [Immunol Invest] 2011; Vol. 40 (6), pp. 581-96. Date of Electronic Publication: 2011 Apr 21. - Publication Year :
- 2011
-
Abstract
- The present study was performed to investigate the effects of dimethylfumarate (DMF) and methylhydrogen fumarate (MHF) on the cytokine pattern of peripheral blood mononuclear cells (PBMCs) of multiple sclerosis (MS) patients. The PBMCs from patients and healthy controls were stimulated with myelin basic protein (MBP) or phytohemagglutinin (PHA) and cultured in the presence of DMF and MHF. The percentage of CD4+IL-4+ and CD4+IFN-γ+ cells was determined by means of intracellular cytokine staining. CD4+IL-4+ cells were significantly increased in the presence of DMF and MHF when PBMCs were stimulated by MBP (P < 0.003). The same significant result was obtained by PHA stimulation (P < 0.049). In terms of CD4+IFN-γ+ cells, the percentage of cells did not significantly differ between the cultures stimulated with MBP or PHA in the presence and absence of the drugs. Results of MBP stimulation in control group also showed a significant increase in CD4+IL-4+ cells in the presence of DMF and MHF. In comparison between patient and control groups, no statistically significant changes were observed. In conclusion, both DMF and MHF effectively increased IL-4 production, whereas they did not significantly change IFN-γ level, indicating the role of these drugs in increasing the production of beneficial cytokines such as IL-4.
- Subjects :
- Adolescent
Adult
CD4-Positive T-Lymphocytes cytology
CD4-Positive T-Lymphocytes drug effects
CD4-Positive T-Lymphocytes immunology
CD4-Positive T-Lymphocytes metabolism
Cell Count
Cell Survival drug effects
Dimethyl Fumarate
Female
Humans
Interferon-gamma metabolism
Interleukin-4 metabolism
Leukocytes, Mononuclear cytology
Leukocytes, Mononuclear drug effects
Leukocytes, Mononuclear immunology
Leukocytes, Mononuclear metabolism
Lymphocyte Activation drug effects
Lymphocyte Activation immunology
Lymphocytes cytology
Lymphocytes immunology
Male
Maleates pharmacology
Middle Aged
Multiple Sclerosis immunology
Myelin Basic Protein immunology
Myelin Basic Protein pharmacology
Phytohemagglutinins pharmacology
Th1 Cells cytology
Th1 Cells drug effects
Th1 Cells immunology
Th1 Cells metabolism
Th2 Cells cytology
Th2 Cells drug effects
Th2 Cells immunology
Th2 Cells metabolism
Young Adult
Cytokines metabolism
Fumarates pharmacology
Lymphocytes drug effects
Lymphocytes metabolism
Multiple Sclerosis metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1532-4311
- Volume :
- 40
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Immunological investigations
- Publication Type :
- Academic Journal
- Accession number :
- 21510778
- Full Text :
- https://doi.org/10.3109/08820139.2011.569626