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Effects of substrate composition on the structure of microbial communities in wastewater using fluorescence in situ hybridisation.
- Source :
-
Systematic and applied microbiology [Syst Appl Microbiol] 2011 Jul; Vol. 34 (5), pp. 337-43. Date of Electronic Publication: 2011 Apr 19. - Publication Year :
- 2011
-
Abstract
- The microbial composition in a pulp and paper wastewater aerated lagoon system was analysed using fluorescence in situ hybridisation (FISH) to gain further understanding of the effect of substrate composition on microbial diversity for improved management of wastewater treatment systems. Few experiments have been conducted to tease apart the factors influencing the composition and abundance of certain groups within these wastewaters. Specific probes were used to investigate and enumerate the different bacterial groups present at particular stages through the treatment system over an extended period. Community composition and abundance of specific groups differed through the system however temporal stability was retained despite significant variability in the wastewater. Middle stream wastewater samples were enriched to explore the impact of different carbon/nitrogen/phosphorus (C:N:P) ratios on community composition and provide functionality to groups of micro-organisms within the microbial consortia. Nitrogen and phosphorus conditions did not impact community composition of methanol-fed cultures, which exhibited a dominance of Betaproteobacteria (>75%), namely Methylotrophic bacteria. This was confirmed through 16S rRNA gene sequencing and specific FISH probing, reflecting population observations at the beginning of the treatment system. We conclude that the nutrient and carbon combinations used in the enrichments created an interactive effect, altering the community composition and mimicking the main substrate load in the different stages of the treatment system. Finally, pulp and paper wastewater microbial composition was highly variable across the treatment system but was stable within the time sampled, with the enrichments emulating the substrate loads in the full scale system.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Acetates pharmacology
Alphaproteobacteria drug effects
Alphaproteobacteria genetics
Alphaproteobacteria physiology
Betaproteobacteria drug effects
Betaproteobacteria genetics
Carbon pharmacology
Cloning, Molecular
Gammaproteobacteria drug effects
Gammaproteobacteria genetics
Gammaproteobacteria physiology
Glucose pharmacology
In Situ Hybridization, Fluorescence methods
Methanol pharmacology
Molecular Sequence Data
Nitrogen metabolism
Nitrogen pharmacology
Nitrogen Fixation
Phosphorus pharmacology
Phylogeny
RNA, Ribosomal, 16S genetics
Waste Disposal, Fluid methods
Betaproteobacteria physiology
Microbial Consortia drug effects
Water Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1618-0984
- Volume :
- 34
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Systematic and applied microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21507592
- Full Text :
- https://doi.org/10.1016/j.syapm.2010.10.006