Back to Search
Start Over
Identification of biomarkers for diagnosis and progression of MS by MALDI-TOF mass spectrometry.
- Source :
-
Multiple sclerosis (Houndmills, Basingstoke, England) [Mult Scler] 2011 Jul; Vol. 17 (7), pp. 838-50. Date of Electronic Publication: 2011 Apr 19. - Publication Year :
- 2011
-
Abstract
- Introduction: Body fluid biomarkers for clinical subtyping and monitoring of disease progression are of considerable interest in multiple sclerosis (MS). Proteomics tools are optimal for the unbiased simultaneous detection of large series of peptides and proteins.<br />Objectives: To identify novel candidate biomarkers discriminating patients with MS from patients with other neurological diseases (OND), and for subtyping of relapsing-remitting (RR), secondary progressive (SP) and primary progressive (PP) MS patients using a high-throughput MALDI-TOF-based mass spectrometry method.<br />Methods: Paired cerebrospinal fluid (CSF) and serum samples of 41 RRMS, 30 SPMS, 13 PPMS patients and 25 patients with OND were analysed.<br />Results: Out of a total of 100 detected peptides in CSF and 200 peptides in serum, 11 peptides were differentially regulated in serum and two in CSF between patients with MS and the OND control group. Eleven peptides were differentially regulated in both serum and CSF between relapse-onset MS and PPMS patients. Lastly, four peptides were differentially regulated in serum and two in CSF between RRMS and SPMS patients. Specific peaks regulated in MS were tentatively identified as fragments of secretogranin III and complement C3. The peak intensity of the CSF peptide ion with m/z value 8607.7 correlated to atrophy (r = -0.27, p < 0.005), black hole volumes (r = 0.31, p < 0.008) and total lesion load (r = 0.34, p < 0.003). A serum peptide with m/z value of 872.4 elevated in SPMS correlated to Expanded Disability Status Scale (r = 0.341, p < 0.005) and atrophy (r = -0.286, p < 0.028).<br />Conclusions: Using high-throughput body fluid profiling by MALDI-TOF mass spectrometry, small proteins and peptides were detected as promising candidate biomarkers for diagnosis and disease progression of MS.
- Subjects :
- Adult
Analysis of Variance
Atrophy
Biomarkers blood
Biomarkers cerebrospinal fluid
Blood Proteins analysis
Brain pathology
Chi-Square Distribution
Disability Evaluation
Disease Progression
Female
Humans
Magnetic Resonance Imaging
Male
Middle Aged
Multiple Sclerosis, Chronic Progressive blood
Multiple Sclerosis, Chronic Progressive cerebrospinal fluid
Multiple Sclerosis, Relapsing-Remitting blood
Multiple Sclerosis, Relapsing-Remitting cerebrospinal fluid
Netherlands
Predictive Value of Tests
Prognosis
Reproducibility of Results
Cerebrospinal Fluid Proteins analysis
Multiple Sclerosis, Chronic Progressive diagnosis
Multiple Sclerosis, Relapsing-Remitting diagnosis
Proteomics methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Subjects
Details
- Language :
- English
- ISSN :
- 1477-0970
- Volume :
- 17
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Multiple sclerosis (Houndmills, Basingstoke, England)
- Publication Type :
- Academic Journal
- Accession number :
- 21505015
- Full Text :
- https://doi.org/10.1177/1352458511399614