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Molecular characterization of lapinized classical Swine Fever vaccine strain by full-length genome sequencing and analysis.
- Source :
-
Animal biotechnology [Anim Biotechnol] 2011 Apr; Vol. 22 (2), pp. 111-7. - Publication Year :
- 2011
-
Abstract
- The complete genome of a lapinized classical swine fever virus (CSFV) vaccine strain was amplified into nine overlapping fragments by RT-PCR, and nucleotide sequences were determined. Complete genome sequence alignment and phylogenetic analysis indicated 92.6-98.6% identities at the nucleotide level with other reported CSFV strains and could be grouped into subgroup 1.1 along with other attenuated strains of CSFV. The 5'-UTR demonstrated >97.0% nucleotide similarity with most of vaccine CSFV strains from China. Further, its 3'-UTR sequence indicated a length similar to all the CSFV strains from China with >98.0% nucleotide similarity, although high length heterogeneity of 3'-UTR was reported among different CSFV strains. There was 12 nt (TTTTCTTTTTTT) insertion in 3'-UTR similar to other reported attenuated vaccine strains. However, secondary structure of 3'-UTR indicated that Indian CSFV strain requires further passage to obtain a 3'-UTR structure similar to most of the attenuated strains.
- Subjects :
- 3' Untranslated Regions genetics
5' Untranslated Regions genetics
Animals
Base Sequence
China
Classical Swine Fever genetics
Genome, Viral
India
Nucleic Acid Conformation
Nucleotides genetics
Phylogeny
RNA, Viral genetics
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Swine
Viral Vaccines classification
Classical Swine Fever virology
Classical Swine Fever Virus classification
Classical Swine Fever Virus genetics
Vaccines, Attenuated genetics
Viral Vaccines genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2378
- Volume :
- 22
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Animal biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 21500112
- Full Text :
- https://doi.org/10.1080/10495398.2011.566472