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Characterization of apoptotic changes induced by yessotoxin in the Bel7402 human hepatoma cell line.
- Source :
-
Molecular medicine reports [Mol Med Rep] 2011 May-Jun; Vol. 4 (3), pp. 547-52. Date of Electronic Publication: 2011 Mar 14. - Publication Year :
- 2011
-
Abstract
- The apoptotic effects of yessotoxin (YTX) on Bel7402 human hepatoma cells have been evaluated by the combination of several methods, including optical microscopy, Hoechst 33342 staining and DNA gel electrophoresis. Rhodamine 123 staining has also been used to investigate changes in mitochondrial transmembrane potential. The results indicated that YTX has negative effects on human liver cells and induces apoptosis. Furthermore, changes in calcium concentrations were analyzed using the fluorescence probe Fluo-3 AM. The results showed that intracellular calcium concentrations increased after treatment with YTX.
- Subjects :
- Calcium metabolism
Carcinoma, Hepatocellular enzymology
Caspase 3 metabolism
Cell Line, Tumor
Cell Nucleus drug effects
Cell Nucleus metabolism
Cell Proliferation drug effects
Cell Shape drug effects
DNA Fragmentation drug effects
Drug Screening Assays, Antitumor
Enzyme Activation drug effects
Humans
Intracellular Space drug effects
Intracellular Space metabolism
Liver Neoplasms enzymology
Membrane Potential, Mitochondrial drug effects
Mollusk Venoms
Okadaic Acid pharmacology
Apoptosis drug effects
Carcinoma, Hepatocellular pathology
Liver Neoplasms pathology
Oxocins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-3004
- Volume :
- 4
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Molecular medicine reports
- Publication Type :
- Academic Journal
- Accession number :
- 21468606
- Full Text :
- https://doi.org/10.3892/mmr.2011.452