Imaging mass spectrometry reveals characteristic changes in triglyceride and phospholipid species in regenerating mouse liver.

Authors :: Miyamura N
Nakamura T
Goto-Inoue N
Zaima N
Hayasaka T
Yamasaki T
Terai S
Sakaida I
Setou M
Nishina H
Source :: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2011 Apr 29; Vol. 408 (1), pp. 120-5. Date of Electronic Publication: 2011 Apr 02.
Publication Year :: 2011
Abstract: After partial hepatectomy (PH), regenerating liver accumulates unknown lipid species. Here, we analyzed lipids in murine liver and adipose tissues following PH by thin-layer chromatography (TLC), imaging mass spectrometry (IMS), and real-time RT-PCR. In liver, IMS revealed that a single TLC band comprised major 19 TG species. Similarly, IMS showed a single phospholipid TLC band to be major 13 species. In adipose tissues, PH induced changes to expression of genes regulating lipid metabolism. Finally, IMS of phosphatidylcholine species demonstrated distribution gradients in lobules that resembled hepatic zonation. IMS is thus a novel and power tool for analyzing lipid species with high resolution.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)

Subjects :: Animals
Chromatography, Thin Layer
Female
Liver metabolism
Mice
Mice, Inbred C57BL
Phospholipids metabolism
Triglycerides metabolism
Liver chemistry
Liver Regeneration
Phospholipids analysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods
Triglycerides analysis

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