A more efficient method to generate integration-free human iPS cells.

Authors :: Okita K
Matsumura Y
Sato Y
Okada A
Morizane A
Okamoto S
Hong H
Nakagawa M
Tanabe K
Tezuka K
Shibata T
Kunisada T
Takahashi M
Takahashi J
Saji H
Yamanaka S
Source :: Nature methods [Nat Methods] 2011 May; Vol. 8 (5), pp. 409-12. Date of Electronic Publication: 2011 Apr 03.
Publication Year :: 2011
Abstract: We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.