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Regulation of human cytotoxic T lymphocyte development by IL-7.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1990 Oct 15; Vol. 145 (8), pp. 2415-20. - Publication Year :
- 1990
-
Abstract
- The effects of IL-7 on the generation of human CTL in alloantigen-, virus-, and lectin-stimulated systems were examined. Addition of IL-7 at the onset of cultures resulted in marked (up to 80-fold) augmentation of cytotoxicity accompanied by smaller (1.5- to 4-fold) increases in total lymphocyte number. Studies of CTL development in purified lectin-stimulated CD8+ T cell populations demonstrated that IL-7 could act directly on the CD8+ lymphocyte subset to augment cytotoxicity. In MLC, the IL-7-induced enhancement of cytotoxicity was found to be mediated primarily by the CD8+ subpopulation of lymphocytes. Late addition of IL-7 (day 5 of 7) resulted in an increase in cytolytic activity that was associated with little or no increase in total or activated CD8+ lymphocyte number indicating that IL-7 may act as a differentiation factor for human CTL. A role for endogenous IL-7 in CTL development was suggested by the observation that addition of neutralizing antiserum to IL-7 to MLC at initiation (or 5 days thereafter) resulted in decreased levels of cytotoxicity. These results indicate that IL-7 can exert major up-regulatory effects on human CTL development and suggest that these effects are both proliferative and differentiative.
- Subjects :
- Antigens, Differentiation, T-Lymphocyte analysis
CD8 Antigens
Concanavalin A pharmacology
Dose-Response Relationship, Drug
Esterases metabolism
Humans
Immunity, Cellular
Immunologic Memory
In Vitro Techniques
Influenza A virus immunology
Interleukin-7 pharmacology
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Time Factors
Cytotoxicity, Immunologic drug effects
Interleukin-7 physiology
T-Lymphocytes, Cytotoxic physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 145
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 2145359