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Responsive microgrooves for the formation of harvestable tissue constructs.
- Source :
-
Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2011 May 03; Vol. 27 (9), pp. 5671-9. Date of Electronic Publication: 2011 Mar 30. - Publication Year :
- 2011
-
Abstract
- Given its biocompatibility, elasticity, and gas permeability, poly(dimethylsiloxane) (PDMS) is widely used to fabricate microgrooves and microfluidic devices for three-dimensional (3D) cell culture studies. However, conformal coating of complex PDMS devices prepared by standard microfabrication techniques with desired chemical functionality is challenging. This study describes the conformal coating of PDMS microgrooves with poly(N-isopropylacrylamide) (PNIPAAm) by using initiated chemical vapor deposition (iCVD). These microgrooves guided the formation of tissue constructs from NIH-3T3 fibroblasts that could be retrieved by the temperature-dependent swelling property and hydrophilicity change of the PNIPAAm. The thickness of swollen PNIPAAm films at 24 °C was approximately 3 times greater than at 37 °C. Furthermore, PNIPAAm-coated microgroove surfaces exhibit increased hydrophilicity at 24 °C (contact angle θ = 30° ± 2) compared to 37 °C (θ = 50° ± 1). Thus PNIPAAm film on the microgrooves exhibits responsive swelling with higher hydrophilicity at room temperature, which could be used to retrieve tissue constructs. The resulting tissue constructs were the same size as the grooves and could be used as modules in tissue fabrication. Given its ability to form and retrieve cell aggregates and its integration with standard microfabrication, PNIPAAm-coated PDMS templates may become useful for 3D cell culture applications in tissue engineering and drug discovery.
- Subjects :
- Acrylamides chemistry
Acrylic Resins
Adsorption
Animals
Cattle
Cell Adhesion drug effects
Dimethylpolysiloxanes pharmacology
Hydrophobic and Hydrophilic Interactions
Mice
Microfluidic Analytical Techniques
NIH 3T3 Cells
Polymers chemistry
Serum Albumin, Bovine chemistry
Temperature
Tissue Engineering
Tissue and Organ Harvesting
Volatilization
Dimethylpolysiloxanes chemistry
Microtechnology methods
Tissue Scaffolds chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5827
- Volume :
- 27
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Langmuir : the ACS journal of surfaces and colloids
- Publication Type :
- Academic Journal
- Accession number :
- 21449596
- Full Text :
- https://doi.org/10.1021/la200183x