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Guidance for understanding solubility as a limiting factor for selecting the upper test concentration in the OECD in vitro micronucleus assay test guideline no. 487.
- Source :
-
Mutation research [Mutat Res] 2011 May 18; Vol. 722 (1), pp. 89-90. Date of Electronic Publication: 2011 Apr 03. - Publication Year :
- 2011
-
Abstract
- The OECD guideline for the in vitro mammalian cell micronucleus test (OECD 487) was recently adopted in July 22, 2010. Since its publication, it has become apparent that the guidance for testing chemicals where solubility is a limiting factor can be interpreted in a variety of ways. In this communication, we provide clarification for testing insoluble chemicals. The intent of the OECD 487 guideline is for the high dose to be the lowest precipitating concentration even if toxicity occurs above the solubility limit in tissue culture medium. Examination of precipitation can be done by the unaided eye or microscopically. Precipitation is examined at the onset or end of treatment, with the intent to identify precipitate present during treatment.<br /> (2011 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 0027-5107
- Volume :
- 722
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Mutation research
- Publication Type :
- Academic Journal
- Accession number :
- 21447401
- Full Text :
- https://doi.org/10.1016/j.mrgentox.2011.03.007