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[Transgenic tomato plants expressing recA and NLS-recA-licBM3 genes as a model for studying meiotic recombination].
- Source :
-
Genetika [Genetika] 2010 Dec; Vol. 46 (12), pp. 1635-44. - Publication Year :
- 2010
-
Abstract
- Homologous DNA recombination in eukaryotes is necessary to maintain genome stability and integrity and for correct chromosome segregation and formation of new haplotypes in meiosis. At the same time, genetic determination and nonrandomness of meiotic recombination restrict the introgression of genes and generation of unique genotypes. As one of the approaches to study and induce meiotic recombination in plants, it is recommended to use the recA gene of Escherichia coli. It is shown that the recA and NLS-recA-licBM3 genes have maternal inheritance and are expressed in the progeny of transgenic tomato plants. Plants expressing recA or NLS-recA-licBM3 and containing one T-DNA insertion do not differ in pollen fertility from original nontransgenic forms and can therefore be used for comparative studies of the effect of bacterial recombinases on meiotic recombination between linked genes.
- Subjects :
- Bacterial Proteins genetics
Clostridium thermocellum enzymology
Genes, Reporter
Glycoside Hydrolases genetics
Solanum lycopersicum genetics
Solanum lycopersicum metabolism
Nuclear Localization Signals
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Plants, Genetically Modified physiology
Rec A Recombinases genetics
Recombinant Fusion Proteins genetics
Recombination, Genetic
Bacterial Proteins biosynthesis
Glycoside Hydrolases biosynthesis
Solanum lycopersicum physiology
Meiosis
Rec A Recombinases biosynthesis
Recombinant Fusion Proteins biosynthesis
Subjects
Details
- Language :
- Russian
- ISSN :
- 0016-6758
- Volume :
- 46
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Genetika
- Publication Type :
- Academic Journal
- Accession number :
- 21434416