[Study of putative periodontal pathogens colonies in type 2 diabetes with chronic periodontitis].

Unlabelled: PPURPOSE: To study the permanent planting of periodontal pathogens in the subgingival plaque of periodontitis patients with type 2 diabetes, and evaluate role of periodontal pathogens in chronic periodontitis and type 2 diabetes.
Methods: One hundred and twenty subgingival plaque samples were collected from 90 severe periodontitis and 30 healthy periodontium patients with type 2 diabetes. Porphyromonas gingivalis (Pg), Aggregatibacer actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Prevotella intermedia (Pi), Tannerella forsythia (Tf) and Treponema denticola (Td) were taken as aim pathogens, polymerase chain reaction (PCR) detection was used to determine the prevalence of Pg, Aa, Fn, Pi, Tf, Td in the subgingival plaque samples.The average gradation(AVG)of positive band in the agarose gel electrophoresis was analyzed to determine the proportion of Pg, Aa, Fn, Pi, Tf, Td in the subgingival plaque. Chi-square test, t test and Pearson correlation were used to analyze the relationship between the periodontal pathogens and blood glucose and blood lipid.
Results: The level of triglyceridey(TC) in Tf positive subjects was significantly higher than that in Tf negative subjects, the proportion of Tf in subgingival plaque was positively correlated with the value of fasting blood glucose(FBG). The proportion of Pg was positively correlated with the level of TC. The proportion of Pi was negatively correlated with the value of FBG and TC.
Conclusion: Type 2 diabetes can affect the permanent planting of some putative periodontal pathogens. Supported by National"11th Five Year" Science and Technology Supporting Project (Grant No. 2007BAI18B02) and Science and Technology Research Plan of Liaoning Province(Grant No. 2007225007).