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Restraint stress and stress hormones significantly impact T lymphocyte migration and function through specific alterations of the actin cytoskeleton.
- Source :
-
Brain, behavior, and immunity [Brain Behav Immun] 2011 Aug; Vol. 25 (6), pp. 1187-96. Date of Electronic Publication: 2011 Mar 21. - Publication Year :
- 2011
-
Abstract
- Stress triggers complex response mechanisms designed to recognize and adapt to perturbations in homeostasis. The immune system is highly responsive to stress, although the complete mechanisms linking stress and immune mediators including T lymphocytes, are not fully understood. Stress exerts its effects on immune effectors through two primary pathways: the sympathetic-adrenal-medullary pathway, and the hypothalamic-pituitary-adrenal pathway which modulate adaptive immunity and lymphocyte migration. In this report we show that stress via release of stress hormones induces early T cell activation and greatly impacts the cytoskeleton by modulating numerous actin-regulating proteins. In particular, proteomic profiling revealed significant decreases in numerous key actin-binding proteins including moesin. Although confocal microscopy showed that moesin and actin were uniformly distributed on the surface of resting T cells, a remarkable polarization and redistribution of moesin and actin was observed following treatment with stress hormones with moesin localizing at the distal pole complex. In addition, the alteration in moesin localization and eventual decrease in expression were accompanied by a loss of CD43; a receptor involved in negatively regulating T cell activation. In conclusion, we have defined a novel molecular mechanism whereby stress hormones negatively impact T cell activation and migration through regulation of key cytoskeletal and plasma membrane factors.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Actins biosynthesis
Actins genetics
Animals
Antigens, CD biosynthesis
Antigens, CD genetics
Antigens, Differentiation, T-Lymphocyte biosynthesis
Antigens, Differentiation, T-Lymphocyte genetics
Catecholamines physiology
Cell Division drug effects
Cell Movement drug effects
Cells, Cultured immunology
Cells, Cultured metabolism
Cells, Cultured ultrastructure
Cytoskeleton metabolism
Female
Glucocorticoids physiology
Ionomycin pharmacology
Lectins, C-Type biosynthesis
Lectins, C-Type genetics
Leukosialin analysis
Lipopolysaccharides pharmacology
Lymphocyte Activation drug effects
Mice
Mice, Inbred BALB C
Microfilament Proteins biosynthesis
Microfilament Proteins genetics
Proteomics
Stress, Physiological physiology
Stress, Psychological physiopathology
T-Lymphocytes drug effects
T-Lymphocytes metabolism
T-Lymphocytes ultrastructure
Tetradecanoylphorbol Acetate pharmacology
Cytoskeleton ultrastructure
Restraint, Physical adverse effects
Stress, Physiological immunology
Stress, Psychological immunology
T-Lymphocytes immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-2139
- Volume :
- 25
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Brain, behavior, and immunity
- Publication Type :
- Academic Journal
- Accession number :
- 21426930
- Full Text :
- https://doi.org/10.1016/j.bbi.2011.03.009