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Induction of transplantation tolerance in mice across major histocompatibility barrier by using allogeneic thymus transplantation and total lymphoid irradiation.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1990 Jul 15; Vol. 145 (2), pp. 499-504. - Publication Year :
- 1990
-
Abstract
- The use of allogeneic thymus transplantation as a means of inducing tolerance across MHC barriers was investigated in thymectomized, total lymphoid irradiated BALB/c mice. In 90% of the animals long term outgrowth of histologically normal C57BL thymus grafts was observed. None of the latter animals was chimeric. All thymus graft-bearing mice showed specific nonresponsiveness for C57BL MHC Ag in mixed lymphocyte reaction and cell-mediated lympholysis. Spleen cells of the C57BL thymus-bearing mice were unable to induce lethal graft-vs-host disease in neonatal (BALB/c X C57BL) F1 mice but provoked a vigorous graft-vs-host disease reaction in (BALB/c x C3H) F1 neonates. Tolerant mice permanently accepted C57BL heart and pancreas grafts, but all rejected C3H grafts. Induction of tolerance of BALB/c pre-T cells through allogeneic thymus graft and/or specific suppressor cells seems to be involved. The present model offers new opportunities to study thymocyte maturation in a fully allogeneic environment and may yield applications for clinical organ transplantation.
- Subjects :
- Animals
Antigens, Differentiation, T-Lymphocyte analysis
Cell Differentiation
Cytotoxicity, Immunologic
Graft vs Host Reaction
Lymphatic System radiation effects
Lymphocyte Culture Test, Mixed
Major Histocompatibility Complex
Mice
Mice, Inbred Strains
Pancreas Transplantation immunology
Radiation, Ionizing
Spleen immunology
T-Lymphocytes immunology
T-Lymphocytes, Regulatory immunology
Thymectomy
Thymus Gland cytology
Thymus Gland immunology
Immune Tolerance radiation effects
Thymus Gland transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 145
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 2142180