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Co-pathological connected primary neurons in a microfluidic device for Alzheimer studies.
- Source :
-
Biotechnology and bioengineering [Biotechnol Bioeng] 2011 Sep; Vol. 108 (9), pp. 2241-5. Date of Electronic Publication: 2011 Mar 21. - Publication Year :
- 2011
-
Abstract
- This communication presents a novel experimental model for Alzheimer studies, where connected primary neurons were set into subtend, co-pathological states. Cortical neurons were cultured in two separated cell compartments in a microfluidic device. A neurite network was generated in a main channel through the neurite outgrowth from both cell compartments. A gradient of okadaic acid (OA) is generated over this neurite network by perfusion. OA is a phosphatase inhibitor that induces hyperphosphorylation of Tau proteins, a major hallmark in Alzheimer disease. The local OA treatment resulted in a connected "diseased" and "healthy" cell population. Anti-phosphorylated tau (Ser262) staining confirmed different states of phosphorylated Tau proteins, and synapthophysin staining the connection of "healthy" and "diseased" cells. Here, we present a novel in vitro model that opens the possibility to study cellular and molecular propagation mechanisms in neurodegeneration, in Tauopathies (as e.g., in Alzheimer), as well as simultaneous drug effects on connected healthy and diseased cell populations.<br /> (Copyright © 2011 Wiley Periodicals, Inc.)
- Subjects :
- Alzheimer Disease metabolism
Animals
Cells, Cultured
Cerebral Cortex metabolism
Cerebral Cortex pathology
Cytological Techniques methods
Nerve Degeneration pathology
Neurons metabolism
Okadaic Acid
Phosphorylation
Rats
Rats, Wistar
tau Proteins metabolism
Alzheimer Disease pathology
Cytological Techniques instrumentation
Microfluidic Analytical Techniques instrumentation
Models, Neurological
Neurons pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1097-0290
- Volume :
- 108
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Biotechnology and bioengineering
- Publication Type :
- Report
- Accession number :
- 21391208
- Full Text :
- https://doi.org/10.1002/bit.23128