Back to Search
Start Over
[Susceptibility of M. tuberculosis to antituberculosis drugs as determined by two methods, in Sucre state, Venezuela].
- Source :
-
Investigacion clinica [Invest Clin] 2010 Dec; Vol. 51 (4), pp. 445-55. - Publication Year :
- 2010
-
Abstract
- The objective of this study was to evaluate the resistance to isoniazid (INH), rifampicin (RIF), streptomycin (STR) and ethambutol (EMB), with the Canetti's proportions method (PM) and the nitrate reductase assay (NRA) of 59 clinical strains of Mycobacterium tuberculosis, isolated in the period of august 2005 to december 2006, in Sucre state, Venezuela. Primary and acquired drug resistance was 6.3% and 14.3%, respectively. Only one strain was found to be multidrug resistant (MDR). The overall agreement between the NRA and PM was 100% for INH, RIF and EMB, and 96% for STR. The time to obtain results was 10 to 14 days for the NRA, compared to 42 days for the PM. The NRA was easy to perform and therefore represents a useful tool for rapid and accurate determination of drug-resistant M. tuberculosis. The sequence of the rpoB gene of the RIF resistant strain demonstrated a never described mutation (change in the codon 456; TCG > CAG) in the hypervariable region of 81 base pairs where most of the mutations of the RIF resistant strains have been reported. Comparison of our results with those of the last resistance prevalence study carried out in the years 1998-1999, shows a decrease in the studied area.
- Subjects :
- Bacterial Proteins analysis
Bacterial Proteins genetics
Base Sequence
DNA-Directed RNA Polymerases
Drug Resistance, Multiple, Bacterial genetics
Ethambutol pharmacology
Humans
Isoniazid pharmacology
Molecular Sequence Data
Morbidity trends
Mutation, Missense
Mycobacterium tuberculosis enzymology
Mycobacterium tuberculosis genetics
Nitrate Reductase analysis
Point Mutation
Prevalence
Rifampin pharmacology
Sequence Alignment
Sequence Homology, Nucleic Acid
Sputum microbiology
Streptomycin pharmacology
Tuberculosis epidemiology
Tuberculosis, Multidrug-Resistant epidemiology
Tuberculosis, Multidrug-Resistant microbiology
Venezuela epidemiology
Antitubercular Agents pharmacology
Drug Resistance, Microbial
Microbial Sensitivity Tests methods
Mycobacterium tuberculosis drug effects
Tuberculosis microbiology
Subjects
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0535-5133
- Volume :
- 51
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Investigacion clinica
- Publication Type :
- Academic Journal
- Accession number :
- 21365873