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Differential changes in expression of intestinal antimicrobial peptide genes during Ascaris lumbricoides infection in Zambian adults do not respond to helminth eradication.
- Source :
-
The Journal of infectious diseases [J Infect Dis] 2011 May 15; Vol. 203 (10), pp. 1464-73. Date of Electronic Publication: 2011 Feb 28. - Publication Year :
- 2011
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Abstract
- Background: Intestinal helminthiasis modulates immune responses to vaccines and environmental allergens. To explore the impact on intestinal host defense, we assessed expression of antimicrobial peptide genes, together with T cell subset markers and cytokines, in patients with ascariasis before and after treatment.<br />Methods: Case patients (n = 27) and control subjects (n = 44) underwent enteroscopy for collection of jejunal biopsy specimens, which were used in quantitative, real-time reverse-transcription polymerase chain reaction for a range of host defense genes; blood samples were also analyzed simultaneously.<br />Results: The level of gene expression (mRNA) of HD5, hBD1, and LL-37 was lower in case patients than in control subjects, and the level of expression of HD6 was increased. However, after successful eradication, there was no trend to values seen in control subjects. Helminthiasis was associated with increased intestinal expression of the Th1 genes T-bet and interferon-γ. In peripheral blood mononuclear cells (PBMCs), a mixed profile of T cell markers and cytokines was increased. Ascaris-induced down-regulation of HD5 was observed in individuals with higher RORγt expression in PBMCs, but we found no evidence that this was mediated by circulating interleukin-22.<br />Conclusions: Human ascariasis was associated with changes in antimicrobial peptide gene expression and immunological markers. Such changes may have implications for susceptibility to infectious disease and responsiveness to oral vaccines in tropical populations.
- Subjects :
- Adult
Animals
Antimicrobial Cationic Peptides genetics
Biomarkers
Case-Control Studies
Cytokines metabolism
Female
Humans
Interferon-gamma genetics
Interferon-gamma metabolism
Intestinal Mucosa metabolism
Leukocytes, Mononuclear
Male
Middle Aged
Nuclear Receptor Subfamily 1, Group F, Member 3 genetics
Nuclear Receptor Subfamily 1, Group F, Member 3 metabolism
T-Box Domain Proteins genetics
T-Box Domain Proteins metabolism
T-Lymphocyte Subsets physiology
Young Adult
Zambia epidemiology
Anthelmintics therapeutic use
Antimicrobial Cationic Peptides metabolism
Ascariasis drug therapy
Ascariasis metabolism
Ascaris lumbricoides
Gene Expression Regulation physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1537-6613
- Volume :
- 203
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The Journal of infectious diseases
- Publication Type :
- Academic Journal
- Accession number :
- 21357944
- Full Text :
- https://doi.org/10.1093/infdis/jir035