Back to Search
Start Over
Cutting edge: vasostatin-1-derived peptide ChgA29-42 is an antigenic epitope of diabetogenic BDC2.5 T cells in nonobese diabetic mice.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2011 Apr 01; Vol. 186 (7), pp. 3831-5. Date of Electronic Publication: 2011 Feb 28. - Publication Year :
- 2011
-
Abstract
- Mechanistic and therapeutic insights in autoimmune diabetes would benefit from a more complete identification of relevant autoantigens. BDC2.5 TCR transgenic NOD mice express transgenes for TCR Vα1 and Vβ4 chains from the highly diabetogenic BDC2.5 CD4(+) T cell clone, which recognizes pancreatic β cell membrane Ags presented by NOD I-A(g7) MHC class II molecules. The antigenic epitope of BDC2.5 TCR is absent in β cells that do not express chromogranin A (ChgA) protein. However, characterization of the BDC2.5 epitope in ChgA has given inconclusive results. We have now identified a ChgA29-42 peptide within vasostatin-1, an N-terminal natural derivative of ChgA as the BDC2.5 TCR epitope. Having the necessary motif for binding to I-A(g7), it activates BDC2.5 T cells and induces an IFN-γ response. More importantly, adoptive transfer of naive BDC2.5 splenocytes activated with ChgA29-42 peptide transferred diabetes into NOD/SCID mice.
- Subjects :
- Adoptive Transfer
Amino Acid Sequence
Animals
Antigen Presentation immunology
Cell Proliferation
Cells, Cultured
Chromogranin A administration & dosage
Chromogranin A metabolism
Diabetes Mellitus, Type 1 pathology
Epitopes, T-Lymphocyte administration & dosage
Epitopes, T-Lymphocyte metabolism
Humans
Islets of Langerhans immunology
Islets of Langerhans pathology
Lymphocyte Activation immunology
Mice
Mice, Inbred NOD
Mice, SCID
Mice, Transgenic
Molecular Sequence Data
Chromogranin A immunology
Diabetes Mellitus, Type 1 immunology
Diabetes Mellitus, Type 1 metabolism
Epitopes, T-Lymphocyte immunology
Peptide Fragments immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 186
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 21357258
- Full Text :
- https://doi.org/10.4049/jimmunol.1003617