Back to Search
Start Over
Mouse 3T3 fibroblasts under the influence of fibroblasts isolated from stroma of human basal cell carcinoma acquire properties of multipotent stem cells.
- Source :
-
Biology of the cell [Biol Cell] 2011 May; Vol. 103 (5), pp. 233-48. - Publication Year :
- 2011
-
Abstract
- Background Information: Multipotent mesenchymal stem cells can participate in the formation of a microenvironment stimulating the aggressive behaviour of cancer cells. Moreover, cells exhibiting pluripotent ESC (embryonic stem cell) markers (Nanog and Oct4) have been observed in many tumours. Here, we investigate the role of cancer-associated fibroblasts in the formation of stem cell supporting properties of tumour stroma. We test the influence of fibroblasts isolated from basal cell carcinoma on mouse 3T3 fibroblasts, focusing on the expression of stem cell markers and plasticity in vitro by means of microarrays, qRT-PCR (quantitative real-time PCR) and immunohistochemistry.<br />Results: We demonstrate the biological activity of the cancer stromal fibroblasts by influencing the 3T3 fibroblasts to express markers such as Oct4, Nanog and Sox2 and to show differentiation potential similar to mesenchymal stem cells. The role of growth factors such as IGF2 (insulin-like growth factor 2), FGF7 (fibroblast growth factor 7), LEP (leptin), NGF (nerve growth factor) and TGFβ (transforming growth factor β), produced by the stromal fibroblasts, is established to participate in their bioactivity. Uninduced 3T3 do not express the stem cell markers and show minimal differentiation potential.<br />Conclusions: Our observations indicate the pro-stem cell activity of cancer-associated fibroblasts and underline the role of epithelial-mesenchymal interaction in tumour biology.
- Subjects :
- 3T3 Cells
Animals
Biomarkers, Tumor metabolism
Cell Differentiation
Cell Separation
Cells, Cultured
Coculture Techniques
Epithelial-Mesenchymal Transition
Humans
Immunohistochemistry
Keratinocytes cytology
Mesenchymal Stem Cells metabolism
Mesenchymal Stem Cells pathology
Mice
Multipotent Stem Cells pathology
Phenotype
Reverse Transcriptase Polymerase Chain Reaction
Carcinoma, Basal Cell pathology
Fibroblasts metabolism
Fibroblasts pathology
Multipotent Stem Cells metabolism
Stromal Cells metabolism
Stromal Cells pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1768-322X
- Volume :
- 103
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 21355851
- Full Text :
- https://doi.org/10.1042/BC20100113