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Quantitative determination of 10 phenylpropanoid and lignan compounds in Lancea tibetica by high-performance liquid chromatography with UV detection.
- Source :
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Planta medica [Planta Med] 2011 Sep; Vol. 77 (13), pp. 1562-6. Date of Electronic Publication: 2011 Feb 23. - Publication Year :
- 2011
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Abstract
- An HPLC method was developed for simultaneous determination of one phenylpropanoid glycoside, verbascoside (1), and nine lignans, including lantibeside (2), phillyrin (3), lantibeside B (4), lantibeside C (5), tibeticoside A (6), styraxjaponoside C (7), sylvatesmin (8), (+)-piperitol (9), and horsfieldin (10), from the Tibetan medicinal plant Lancea tibetica Hook. F. et Thoms. The analysis was performed within 45 min. The extraction method was optimized with different solvent systems. The HPLC method was validated for linearity, repeatability, accuracy, limits of detection, and limits of quantification. The limits of detection and limits of quantification of 10 analytes were found to be less than 0.1 and 0.5 µg/mL, respectively. The RSD for intra- and inter-day analyses was less than 4.2 %, and the recovery efficiency was 90-105 %. The method was used to analyze different populations of L. tibetica collected in China. HPLC profiles showed that the concentrations of analytes were different in samples collected from different areas of China. Verbascoside was the dominant component in three out of five plant samples; compounds 2, 3, 6, and 8 accounted for over 62 % yields in total lignan contents. The method is useful for identification, quality assurance, and quality control of L. tibetica and its related products.<br /> (© Georg Thieme Verlag KG Stuttgart · New York.)
- Subjects :
- Calibration
China
Glucosides chemistry
Lignans chemistry
Medicine, Tibetan Traditional
Phenols chemistry
Plant Extracts chemistry
Quality Control
Ultraviolet Rays
Chromatography, High Pressure Liquid methods
Glucosides isolation & purification
Lignans isolation & purification
Phenols isolation & purification
Plant Extracts isolation & purification
Scrophulariaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1439-0221
- Volume :
- 77
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Planta medica
- Publication Type :
- Academic Journal
- Accession number :
- 21347996
- Full Text :
- https://doi.org/10.1055/s-0030-1270833