Back to Search
Start Over
Extracellular DNA is essential for maintaining Bordetella biofilm integrity on abiotic surfaces and in the upper respiratory tract of mice.
- Source :
-
PloS one [PLoS One] 2011 Feb 11; Vol. 6 (2), pp. e16861. Date of Electronic Publication: 2011 Feb 11. - Publication Year :
- 2011
-
Abstract
- Bacteria form complex and highly elaborate surface adherent communities known as biofilms which are held together by a self-produced extracellular matrix. We have previously shown that by adopting a biofilm mode of existence in vivo, the gram negative bacterial pathogens Bordetella bronchiseptica and Bordetella pertussis are able to efficiently colonize and persist in the mammalian respiratory tract. In general, the bacterial biofilm matrix includes polysaccharides, proteins and extracellular DNA (eDNA). In this report, we investigated the function of DNA in Bordetella biofilm development. We show that DNA is a significant component of Bordetella biofilm matrix. Addition of DNase I at the initiation of biofilm growth inhibited biofilm formation. Treatment of pre-established mature biofilms formed under both static and flow conditions with DNase I led to a disruption of the biofilm biomass. We next investigated whether eDNA played a role in biofilms formed in the mouse respiratory tract. DNase I treatment of nasal biofilms caused considerable dissolution of the biofilm biomass. In conclusion, these results suggest that eDNA is a crucial structural matrix component of both in vitro and in vivo formed Bordetella biofilms. This is the first evidence for the ability of DNase I to disrupt bacterial biofilms formed on host organs.
- Subjects :
- Animals
Biofilms drug effects
Bordetella bronchiseptica cytology
Bordetella bronchiseptica drug effects
Bordetella bronchiseptica metabolism
Bordetella pertussis cytology
Bordetella pertussis drug effects
Bordetella pertussis metabolism
Deoxyribonuclease I pharmacology
Extracellular Space drug effects
Female
Hydrodynamics
Mice
Mice, Inbred C57BL
Nasal Septum drug effects
Nasal Septum microbiology
Nasopharynx drug effects
Nasopharynx microbiology
Respiratory System drug effects
Surface Properties
Biofilms growth & development
Bordetella bronchiseptica physiology
Bordetella pertussis physiology
DNA, Bacterial metabolism
Extracellular Space metabolism
Respiratory System microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 6
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 21347299
- Full Text :
- https://doi.org/10.1371/journal.pone.0016861