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Overexpression of HARDY, an AP2/ERF gene from Arabidopsis, improves drought and salt tolerance by reducing transpiration and sodium uptake in transgenic Trifolium alexandrinum L.
- Source :
-
Planta [Planta] 2011 Jun; Vol. 233 (6), pp. 1265-76. Date of Electronic Publication: 2011 Feb 22. - Publication Year :
- 2011
-
Abstract
- Trifolium alexandrinum L. was transformed with the Arabidopsis HARDY gene that belongs to the stress-related AP2/ERF (APETALA2/ethylene responsive element binding factors) superfamily of transcription factors. The fresh weights of the transgenic lines L2 and L3 were improved by 42 and 55% under drought stress and by 38 and 95% under salt stress compared to the wild type, respectively. The dry weights were similarly improved. Overexpression of HARDY improved the instantaneous water use efficiency (WUE) under drought stress by reducing transpiration (E) and under salt stress by improving photosynthesis (A), through reducing Na+ accumulation in leaves, and reducing E. However, HARDY improved the growth of drought-stressed transgenic plants as compared to the wild type by delaying water depletion from soil and preventing rapid decline in A. L2 and L3 had thicker stems and in case of L3, more xylem rows per vascular bundle, which may have made L3 more resistant to lodging in the field. Field performance of L2 and L3 under combined drought and salt stress was significantly better than that of the wild type in terms of fresh and dry weights (40%, 46% and 31%, 40%, respectively). The results provide further evidence for the efficiency of overexpression of a single gene in improving tolerance to abiotic stress under field conditions.
- Subjects :
- Arabidopsis genetics
Arabidopsis metabolism
Arabidopsis Proteins genetics
Biological Transport
Dehydration genetics
Gene Expression Regulation, Plant
Genes, Plant
Genetic Variation
Mutation
Photosynthesis
Plant Leaves metabolism
Plant Stems cytology
Plant Transpiration
Plants, Genetically Modified genetics
Plants, Genetically Modified growth & development
Salinity
Salt Tolerance genetics
Transcription Factor AP-2 genetics
Trifolium genetics
Trifolium growth & development
Water metabolism
Arabidopsis Proteins biosynthesis
Dehydration metabolism
Plants, Genetically Modified metabolism
Sodium Chloride pharmacokinetics
Transcription Factor AP-2 biosynthesis
Trifolium metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-2048
- Volume :
- 233
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Planta
- Publication Type :
- Academic Journal
- Accession number :
- 21340699
- Full Text :
- https://doi.org/10.1007/s00425-011-1382-3