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Interactive role of trauma cytokines and erythropoietin and their therapeutic potential for acute and chronic wounds.
- Source :
-
Rejuvenation research [Rejuvenation Res] 2011 Feb; Vol. 14 (1), pp. 57-66. - Publication Year :
- 2011
-
Abstract
- If controllable, stem cell activation following injury has the therapeutic potential for supporting regeneration in acute or chronic wounds. Human dermally-derived stem cells (FmSCs) were exposed to the cytokines interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) in the presence of erythropoietin (EPO). Cells were cultured under ischemic conditions and phenotypically characterized using flow cytometry. Topical EPO application was performed in three independent clinical wound healing attempts. The FmSCs expressed the receptor for EPO. EPO had a strong inhibitory effect on FmSC growth in the absence of IL-6 and TNF-α. With IL-6, the EPO effects were reversed to that of growth stimulation. TNF-α had the strongest stimulatory effect. In contrast, IL-1β had an inhibitory effect. Topically applied EPO considerably enhanced wound healing and improved wound conditions of acute and chronic wounds. Site specificity of stem cell activation is mediated by IL-6 and TNF-α. In trauma, EPO ceases its inhibitory role and reverts to a clinically relevant boosting function. EPO may be an important therapeutic tool for the topical treatment of acute and chronic wounds.
- Subjects :
- Acute Disease
Adult
Aged
Biomarkers metabolism
Cell Proliferation drug effects
Cells, Cultured
Child, Preschool
Chronic Disease
Cytokines pharmacology
Dermis cytology
Drug Synergism
Erythropoietin pharmacology
Female
Flow Cytometry
Humans
Male
Middle Aged
Phenotype
Receptors, Erythropoietin chemistry
Sequence Analysis, Protein
Stem Cells cytology
Stem Cells drug effects
Stem Cells metabolism
Wounds and Injuries pathology
Cytokines therapeutic use
Erythropoietin therapeutic use
Wounds and Injuries drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8577
- Volume :
- 14
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Rejuvenation research
- Publication Type :
- Academic Journal
- Accession number :
- 21329452
- Full Text :
- https://doi.org/10.1089/rej.2010.1050