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Composition of the bacterial biota in slime developed in two machines at a Canadian paper mill.
- Source :
-
Canadian journal of microbiology [Can J Microbiol] 2011 Feb; Vol. 57 (2), pp. 91-104. - Publication Year :
- 2011
-
Abstract
- During the process of papermaking by pulp and paper plants, a thick and viscous deposits, termed slime, is quickly formed around the paper machines, which can affect the papermaking process. In this study, we explored the composition of the bacterial biota in slime that developed on shower pipes from 2 machines at a Canadian paper mill. Firstly, the composition was assessed for 12 months by DNA profiling with polymerase chain reaction coupled with denaturing gradient gel electrophoresis. Except for short periods (2-3 months), clustered analyses showed that the bacterial composition of the slime varied substantially over the year, with less than 50% similarity between the denaturing gradient gel electrophoresis profiles. Secondly, the screening of 16S rRNA gene libraries derived from 2 slime samples showed that the most abundant bacteria were related to 6 lineages, including Chloroflexi, candidate division OP10, Clostridiales, Bacillales, Burkholderiales, and the genus Deinococcus. Finally, the proportion of 8 bacterial lineages, such as Deinococcus sp., Meiothermus sp., and Chloroflexi, was determined by the Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization in 2 slime samples. The results showed a high proportion of Chloroflexi, Tepidimonas spp., and Schlegelella spp. in the slime samples.
- Subjects :
- Bacteria genetics
Biofouling
Canada
DNA Fingerprinting
DNA, Bacterial genetics
Denaturing Gradient Gel Electrophoresis
Gene Library
In Situ Hybridization, Fluorescence
Paper
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S genetics
Sequence Analysis, DNA
Bacteria classification
Biofilms
Biota
Industrial Waste
Subjects
Details
- Language :
- English
- ISSN :
- 1480-3275
- Volume :
- 57
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Canadian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 21326351
- Full Text :
- https://doi.org/10.1139/w10-109