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Intestinal metabolite compound K of panaxoside inhibits the growth of gastric carcinoma by augmenting apoptosis via Bid-mediated mitochondrial pathway.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2012 Jan; Vol. 16 (1), pp. 96-106. - Publication Year :
- 2012
-
Abstract
- Compound K (20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol, CK), an intestinal bacterial metabolite of panaxoside, has been shown to inhibit tumour growth in a variety of tumours. However, the mechanisms involved are largely unknown. We use human gastric carcinoma cell lines BGC823, SGC7901 and human gastric carcinoma xenograft in nude mice as models to study the mechanisms of CK in gastric cancers. We found that CK significantly inhibits the viabilities of BGC823 and SGC7901 cells in dose- and time-dependent manners. CK-induced BGC823 and SGC7901 cells apoptosis and cell cycle arrest in G2 phase by up-regulation of p21 and down-regulation of cdc2 and cyclin B1. Further studies show that CK induces apoptosis in BGC823 and SGC7901 cells mainly through mitochondria-mediated internal pathway, and that CK induces the translocation of nuclear Bid to mitochondria. Finally, we found that CK effectively inhibited the tumour formation of SGC7901 cells in nude mice. Our studies show that CK can inhibit the viabilities and induce apoptosis of human gastric carcinoma cells via Bid-mediated mitochondrial pathway.<br /> (© 2011 The Authors Journal of Cellular and Molecular Medicine © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd.)
- Subjects :
- Animals
Cell Cycle Checkpoints drug effects
Cell Line, Tumor drug effects
Cell Line, Tumor metabolism
Cell Line, Tumor pathology
Cell Survival drug effects
Female
Ginsenosides pharmacology
Ginsenosides therapeutic use
Humans
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Apoptosis drug effects
BH3 Interacting Domain Death Agonist Protein metabolism
Ginsenosides metabolism
Intestinal Mucosa metabolism
Mitochondria metabolism
Stomach Neoplasms drug therapy
Stomach Neoplasms pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 21323864
- Full Text :
- https://doi.org/10.1111/j.1582-4934.2011.01278.x