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Vaccination with OK-432 followed by TC-1 tumor lysate leads to significant antitumor effects.
- Source :
-
Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2011 Jul; Vol. 18 (7), pp. 687-94. Date of Electronic Publication: 2011 Feb 14. - Publication Year :
- 2011
-
Abstract
- Human papillomavirus (HPV) infects large numbers of women worldwide and is present in more than 99% of all cervical cancer. TC-1 cell is a cell line with high expression of E7 antigen of HPV type 16 and its cell lysate has been demonstrated as an ideal inducer of E7-specific, antitumor immunity. OK-432 (Picibanil), a penicillin-killed Streptococcus pyogenes, has been reported with potent immunomodulation properties in cancer treatment by stimulating the maturation of dendritic cells (DCs) and secretion of Th-1 type cytokines. The current study demonstrated that a protocol to immunize the C57BL/6 mice with OK-432 followed by treatment with TC-1 lysate can generate markedly increased immune responses of E7-specific CD4(+) T cells and a moderate increase of natural killer (NK) cell, as well as a satisfactorily protective and therapeutic antitumor effect by triggering the DCs to prime T cells. Depletion of lymphocyte subset in vivo suggested that the antitumor effects could be dominantly executed by CD8+ T cells and followed by NK cells, and both of these reactions were induced by the generation of robust E7-specific CD4(+) T helper cell response. These findings warrant OK-432 combination with tumor-lysate as an effective and safe vaccine in future clinical application of cervical cancer.
- Subjects :
- Animals
Cell Line, Tumor
Female
Mice
Mice, Inbred C57BL
Papillomavirus Infections immunology
Papillomavirus Infections virology
Picibanil immunology
Uterine Cervical Neoplasms immunology
Uterine Cervical Neoplasms virology
Viral Vaccines immunology
Alphapapillomavirus immunology
Papillomavirus E7 Proteins immunology
Papillomavirus Infections prevention & control
Picibanil pharmacology
Uterine Cervical Neoplasms prevention & control
Viral Vaccines pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1933-7205
- Volume :
- 18
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Reproductive sciences (Thousand Oaks, Calif.)
- Publication Type :
- Academic Journal
- Accession number :
- 21321245
- Full Text :
- https://doi.org/10.1177/1933719110396230