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Rapid and reliable measurement for evaluating directly the reactivity of N-acetylcysteine with glucose degradation products in peritoneal dialysis fluids.
- Source :
-
Analytical chemistry [Anal Chem] 2011 Mar 01; Vol. 83 (5), pp. 1518-22. Date of Electronic Publication: 2011 Feb 07. - Publication Year :
- 2011
-
Abstract
- In this report, we analyzed the reactivity of N-acetyl-L-cysteine (NAC) with glucose degradation products (GDPs) and the stability of NAC in peritoneal dialysis fluids (PDFs) using RP-HPLC and LC-ESI-TOF-MS. NAC reduced the amount of 3,4-dideoxyglucosone-3-ene (3,4-DGE), most toxic among GDPs in PDFs by forming NAC-DGE conjugate under nonenzymatic conditions. NAC was retained as a reduced monomer form in the high-glucose compartment of dual-chambered neutral-pH type PDF, whereas it easily formed a homodimer in an incubation-time-dependent manner in other solutions. The present investigation suggests that NAC can be employed as an adjuvant added into the high-glucose compartment of neutral-pH type PDFs (N-PDF) to reduce GDP-mediated peritoneal membrane failure in patients on long-term peritoneal dialysis (PD) treatment.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 83
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Report
- Accession number :
- 21299245
- Full Text :
- https://doi.org/10.1021/ac200046y