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Correlation between T-cadherin gene expression and aberrant methylation of T-cadherin promoter in human colon carcinoma cells.
- Source :
-
Medical oncology (Northwood, London, England) [Med Oncol] 2012 Jun; Vol. 29 (2), pp. 915-8. Date of Electronic Publication: 2011 Feb 05. - Publication Year :
- 2012
-
Abstract
- Previous researches showed T-cadherin (CDH13) expression was downregulated in colon cancer tissues and was associated with increase of invasive and metastatic potential. This research was to observe the mechanisms responsible for inactivation of T-cadherin gene in colon carcinoma; we investigated the methylation status around the 5' promoter region of T-cadherin gene of Hct116 colon cancer cell line by methylation-specific polymerase chain reaction (MSP), also detected the expression change of T-cadherin mRNA and protein in Hct116 cell line after 5-Aza-CdR treatment by reverse transcriptase polymerase chain reaction and Western blotting, and compared the T-cadherin methylation status with T-cadherin mRNA and protein expression. We found that hypermethylation of T-cadherin was involved in Hct116 cell line, while T-cadherin mRNA and protein expression was almost lost or downregulated in Hct116 cell line. Therefore, methylation of the T-cadherin promoter region was correlated with the loss or downregulation of T-cadherin mRNA and protein expression in Hct116 colon cancer cell line. Treatment of T-cadherin-negative carcinoma cells with the demethylating agent, 5-aza-2'-deoxycytidine, induced re-expression of this gene. Our findings demonstrate that 5' CpG island methylation is common in colon carcinoma and may play an important role in the inaction of T-cadherin. Our results also suggest that demethylation of the T-cadherin gene may be a potential therapeutic strategy for colon carcinoma.
- Subjects :
- Azacitidine analogs & derivatives
Azacitidine pharmacology
Blotting, Western
Decitabine
Humans
RNA, Messenger genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Cadherins genetics
Cadherins metabolism
Colonic Neoplasms genetics
CpG Islands genetics
DNA Methylation
Gene Expression Regulation, Neoplastic
Promoter Regions, Genetic genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1559-131X
- Volume :
- 29
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Medical oncology (Northwood, London, England)
- Publication Type :
- Academic Journal
- Accession number :
- 21298366
- Full Text :
- https://doi.org/10.1007/s12032-011-9836-9