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Pectin engineering to modify product quality in potato.
Pectin engineering to modify product quality in potato.
- Source :
-
Plant biotechnology journal [Plant Biotechnol J] 2011 Oct; Vol. 9 (8), pp. 848-56. Date of Electronic Publication: 2011 Feb 01. - Publication Year :
- 2011
-
Abstract
- Although processed potato tuber texture is an important trait that influences consumer preference, a detailed understanding of tuber textural properties at the molecular level is lacking. Previous work has identified tuber pectin methyl esterase (PME) activity as a potential factor impacting on textural properties, and the expression of a gene encoding an isoform of PME (PEST1) was associated with cooked tuber textural properties. In this study, a transgenic approach was undertaken to investigate further the impact of the PEST1 gene. Antisense and over-expressing potato lines were generated. In over-expressing lines, tuber PME activity was enhanced by up to 2.3-fold; whereas in antisense lines, PME activity was decreased by up to 62%. PME isoform analysis indicated that the PEST1 gene encoded one isoform of PME. Analysis of cell walls from tubers from the over-expressing lines indicated that the changes in PME activity resulted in a decrease in pectin methylation. Analysis of processed tuber texture demonstrated that the reduced level of pectin methylation in the over-expressing transgenic lines was associated with a firmer processed texture. Thus, there is a clear link between PME activity, pectin methylation and processed tuber textural properties.<br /> (© 2011 The Authors. Plant Biotechnology Journal © 2011 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.)
- Subjects :
- Agrobacterium tumefaciens genetics
Carboxylic Ester Hydrolases genetics
Cell Wall metabolism
Enzyme Activation
Food Handling
Gene Expression Regulation, Plant
Genes, Plant
Genetic Vectors genetics
Isoenzymes metabolism
Methylation
Oligonucleotide Array Sequence Analysis
Pectins genetics
Pectins metabolism
Plant Tubers genetics
Plant Tubers metabolism
Plants, Genetically Modified genetics
Plants, Genetically Modified metabolism
Plants, Genetically Modified physiology
Solanum tuberosum metabolism
Solanum tuberosum physiology
Spectroscopy, Fourier Transform Infrared
Surface Properties
Transgenes
Carboxylic Ester Hydrolases metabolism
Gene Expression Regulation, Enzymologic
Genetic Engineering methods
Plant Tubers physiology
Solanum tuberosum genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1467-7652
- Volume :
- 9
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Plant biotechnology journal
- Publication Type :
- Academic Journal
- Accession number :
- 21281424
- Full Text :
- https://doi.org/10.1111/j.1467-7652.2011.00591.x