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Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves.
- Source :
-
Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2011 Mar; Vol. 49 (3), pp. 357-62. Date of Electronic Publication: 2011 Jan 13. - Publication Year :
- 2011
-
Abstract
- Lipid phosphate phosphatases (LPPs, E.C. 3.1.3.4) catalyse the dephosphorylation of diacylglycerol pyrophosphate (DGPP) and phosphatidic acid (PA), which are secondary messengers in abscisic acid (ABA) signalling. In this study, we investigated the effect of ABA on the expression of AtLPP genes as they encode putative ABA-signalling partners. We observed that AtLPP2 expression was down-regulated by ABA and we performed experiments on Atlpp2-2, an AtLPP2 knockout mutant, to determine whether AtLPP2 was involved in ABA signalling. We observed that Atlpp2-2 plantlets contained about twice as much PA as the wild-type Col-0 and exhibited higher PA kinase (PAK) activity than Col-0 plants. In addition, we showed that ABA stimulated diacylglycerol kinase (DGK) activity independently of AtLPP2 activity but that the ABA-stimulation of PAK activity recorded in Col-0 was dependent on AtLPP2. In order to evaluate the involvement of AtLPP2 activity in guard cell function, we measured the ABA sensitivity of Atlpp2-2 stomata. The inhibition of stomatal opening was less sensitive to ABA in Atlpp2-2 than in Col-0. Watered and water-stressed plants of the two genotypes accumulated ABA to the same extent, thus leading us to consider Atlpp2-2 an ABA-signalling mutant. Taken together our observations show that AtLPP2 is a part of ABA signalling and participate to the regulation of stomatal movements.<br /> (Copyright © 2011 Elsevier Masson SAS. All rights reserved.)
- Subjects :
- Abscisic Acid pharmacology
Adaptation, Physiological genetics
Arabidopsis genetics
Arabidopsis Proteins genetics
Diacylglycerol Kinase metabolism
Down-Regulation
Droughts
Gene Expression drug effects
Genotype
Mutation
Phosphatidate Phosphatase genetics
Phosphatidic Acids metabolism
Phosphotransferases (Phosphate Group Acceptor) metabolism
Signal Transduction genetics
Stress, Physiological genetics
Water physiology
Abscisic Acid metabolism
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Gene Expression Regulation, Plant drug effects
Genes, Plant
Phosphatidate Phosphatase metabolism
Plant Stomata physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2690
- Volume :
- 49
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Plant physiology and biochemistry : PPB
- Publication Type :
- Academic Journal
- Accession number :
- 21277215
- Full Text :
- https://doi.org/10.1016/j.plaphy.2011.01.010