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Activation of TREK currents by the neuroprotective agent riluzole in mouse sympathetic neurons.
- Source :
-
The Journal of neuroscience : the official journal of the Society for Neuroscience [J Neurosci] 2011 Jan 26; Vol. 31 (4), pp. 1375-85. - Publication Year :
- 2011
-
Abstract
- Background K2P channels play a key role in stabilizing the resting membrane potential, thereby modulating cell excitability in the central and peripheral somatic nervous system. Whole-cell experiments revealed a riluzole-activated current (I(RIL)), transported by potassium, in mouse superior cervical ganglion (mSCG) neurons. The activation of this current by riluzole, linoleic acid, membrane stretch, and internal acidification, its open rectification and insensitivity to most classic potassium channel blockers, indicated that I(RIL) flows through channels of the TREK [two-pore domain weak inwardly rectifying K channel (TWIK)-related K channel] subfamily. Whole-ganglia and single-cell reverse transcription-PCR demonstrated the presence of TREK-1, TREK-2, and TRAAK (TWIK-related arachidonic acid-activated K(+) channel) mRNA, and the expression of these three proteins was confirmed by immunocytochemistry in mSCG neurons. I(RIL) was enhanced by zinc, inhibited by barium and fluoxetine, but unaffected by quinine and ruthenium red, strongly suggesting that it was carried through TREK-1/2 channels. Consistently, a channel with properties identical with the heterologously expressed TREK-2 was recorded in most (75%) cell-attached patches. These results provide the first evidence for the expression of K2P channels in the mammalian autonomic nervous system, and they extend the impact of these channels to the entire nervous system.
- Subjects :
- Action Potentials drug effects
Animals
Cells, Cultured
Membrane Potentials drug effects
Mice
Neurons physiology
Patch-Clamp Techniques
Potassium Channels, Tandem Pore Domain genetics
RNA, Messenger biosynthesis
Superior Cervical Ganglion cytology
Superior Cervical Ganglion physiology
Neurons drug effects
Neuroprotective Agents pharmacology
Potassium Channels, Tandem Pore Domain biosynthesis
Riluzole pharmacology
Superior Cervical Ganglion drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1529-2401
- Volume :
- 31
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 21273422
- Full Text :
- https://doi.org/10.1523/JNEUROSCI.2791-10.2011