Back to Search
Start Over
RpoE1, an extracytoplasmic function sigma factor, is a repressor of the flagellar system in Brucella melitensis.
- Source :
-
Microbiology (Reading, England) [Microbiology (Reading)] 2011 May; Vol. 157 (Pt 5), pp. 1263-1268. Date of Electronic Publication: 2011 Jan 27. - Publication Year :
- 2011
-
Abstract
- The genome of Brucella melitensis contains genes coding for the sigma factors RpoD, RpoN, RpoH1, RpoH2, RpoE1 and RpoE2. Previously published data show that B. melitensis is flagellated and that an rpoE1 mutant overexpresses the flagellar protein FlgE. In this study, we demonstrate that mutation of rpoE1 causes an overexpression of the flagellar genes fliF, flgE, fliC, flaF and flbT, correlating with the production of a longer filament and thereby demonstrating that RpoE1 acts as a flagellar repressor. Moreover, mutation of rpoE1 increases the promoter activity of the flagellar master regulator ftcR, suggesting that RpoE1 acts upstream of ftcR. Together, these data show that RpoE1 represses the flagellar synthesis and filament length in B. melitensis.
- Subjects :
- Bacterial Proteins genetics
Brucella melitensis genetics
Flagella genetics
Mutation
Protein Transport
Repressor Proteins genetics
Sigma Factor genetics
Bacterial Proteins metabolism
Brucella melitensis metabolism
Flagella metabolism
Gene Expression Regulation, Bacterial
Repressor Proteins metabolism
Sigma Factor metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1465-2080
- Volume :
- 157
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- Microbiology (Reading, England)
- Publication Type :
- Academic Journal
- Accession number :
- 21273248
- Full Text :
- https://doi.org/10.1099/mic.0.044875-0