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DNA barcoding analysis of fish species diversity in four north Greek lakes.
- Source :
-
Mitochondrial DNA [Mitochondrial DNA] 2011 Oct; Vol. 22 Suppl 1, pp. 37-42. Date of Electronic Publication: 2011 Jan 24. - Publication Year :
- 2011
-
Abstract
- Materials and Methods: The present study is the first to apply DNA barcoding on identifying 37 freshwater fish species from the rich Balkan ichthyofauna.<br />Results: The results are highly successful since in most cases barcodes cluster according to species, in agreement with morphological taxonomic studies. This is also evident based on mean conspecific and congeneric Kimura two-parameter distance values. The 5.6-fold difference between these values is lower than previous barcoding studies, possibly due to the restricted samplings and the recent taxonomy reevaluation for several species. A number of species were identified, where future work is needed: For the species Scardinius erythrophthalmus, Perca fluviatilis, and Rutilus rutilus, the divergence values found among conspecific populations could warrant their placement into different species; for Barbus and Rhodeus populations, the reported interspecific distances found were lower than expected; and for Cobitis species, the application of barcoding seems problematic, due to their complicated reproduction.<br />Conclusion: The extension of this work to other Greek or even Balkan freshwater systems should clarify the situation.
- Subjects :
- Animals
Cyprinidae classification
Cyprinidae genetics
DNA Barcoding, Taxonomic standards
DNA, Mitochondrial analysis
DNA, Mitochondrial genetics
Genetic Variation
Greece
Perciformes classification
Perciformes genetics
Sequence Analysis, DNA
Species Specificity
DNA Barcoding, Taxonomic methods
Electron Transport Complex IV genetics
Fishes classification
Fishes genetics
Lakes
Subjects
Details
- Language :
- English
- ISSN :
- 1940-1744
- Volume :
- 22 Suppl 1
- Database :
- MEDLINE
- Journal :
- Mitochondrial DNA
- Publication Type :
- Academic Journal
- Accession number :
- 21261496
- Full Text :
- https://doi.org/10.3109/19401736.2010.542242