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Myoepithelial cell layer integrity in canine mammary carcinoma.
- Source :
-
Journal of comparative pathology [J Comp Pathol] 2011 Jul; Vol. 145 (1), pp. 25-30. Date of Electronic Publication: 2011 Jan 17. - Publication Year :
- 2011
-
Abstract
- The aim of this study was to determine whether the myoepithelial (ME) cell marker calponin could be used to analyze the integrity of the ME cell layer as a means of identifying canine mammary carcinoma in situ. Tissue from 74 canine mammary lesions was evaluated (two dysplasia, eight benign tumours and 64 carcinomas including one carcinoma in situ). The 63 carcinomas included examples of histological grade 1 (n=32), grade 2 (n=23) and grade 3 (n=8). Expression of calponin was determined by immunohistochemistry. The percentage of proliferating cells surrounded by a single layer of calponin-positive cells formed the basis of classification as type I (≥ 90%), type II (70-90%) and type III (≤ 70%). Expression of Ki67 was used to determine the proliferation index (PI). The malignant tumours comprised of an approximately equal mixture of type I, II and III lesions. The two examples of dysplasia, the carcinoma in situ and two thirds of the benign tumours were classified as type I lesions. Some overlap in the level of calponin expression was observed between benign and malignant tumours. Positive correlations between the degree of calponin expression and the type of lesion (i.e. benign versus malignant; R=+0.3, P=0.08) and the histological grade of malignancy (R=+0.54, P=0.000001) were found. A negative correlation between the degree of calponin expression and PI (R=+0.027, P=0.016) was found. The ME cell marker calponin may be used as an aid in the identification of canine carcinoma in situ, but the study of the ME cell layer integrity is not definitive for the diagnosis of malignancy in canine mammary tumours.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Biomarkers, Tumor metabolism
Calcium-Binding Proteins metabolism
Dog Diseases metabolism
Dogs
Epithelial Cells metabolism
Epithelial Cells pathology
Female
Immunohistochemistry
Mammary Neoplasms, Animal metabolism
Microfilament Proteins metabolism
Calponins
Biomarkers, Tumor analysis
Calcium-Binding Proteins analysis
Dog Diseases pathology
Mammary Neoplasms, Animal pathology
Microfilament Proteins analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1532-3129
- Volume :
- 145
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of comparative pathology
- Publication Type :
- Academic Journal
- Accession number :
- 21247585
- Full Text :
- https://doi.org/10.1016/j.jcpa.2010.11.006