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[The estrogenic effect of formononetin and its effect on the expression of rats' atrium estrogen receptors].
- Source :
-
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials [Zhong Yao Cai] 2010 Sep; Vol. 33 (9), pp. 1445-9. - Publication Year :
- 2010
-
Abstract
- Objective: To observe the estrogenic effect of formononetin and its effect on the expressions of atrial estrogen receptor subtypes alpha and beta (ERalpha and ERbeta).<br />Methods: 50 femal rats were randomly divided into five groups: sham group, model group, nilestriol group, formononetin groups of low and high dose. Rats in sham group were cut a piece of fat before closing the abdomen, the others were ovariectomized. Vaginal exfoliated cell were observed from the fifth day to the tenth after operation to test if the model is successful. The sham and model group were given nomal saline in 10 mL/kg by gavage, the remaining three groups were given nilestriol 2.5 mg/(kg x w), low [20 mg/(kg x d) land high dose [100 mg/(kg x d)) of formononetin by gavage respectively. In the 8th week, vaginal exfoliated cell were observed, then decapitated the rats, removed the uterus, weighed and take wright staining microscopy. The relative expressions of ERalpha and ERbeta of right atrium were detected by RT-PCR.<br />Results: The vaginal cells exhibit a change of estrus after had been fed with high dose of formononetin after 8 weeks. Formononetin increase the uterus coefficient and the expression of atrial ERbeta (P < 0.01), but it dose not have any effect on the expression of ERalpha (P > 0.05).<br />Conclusion: Formononetin have estrogenic effect in ovariectomized rats, and it can markedly upregulate the expression of rats' atrial ERbeta.
- Subjects :
- Administration, Oral
Animals
Disease Models, Animal
Estriol administration & dosage
Estriol analogs & derivatives
Estriol pharmacology
Female
Isoflavones administration & dosage
Ovariectomy
Phytoestrogens administration & dosage
Quinestrol analogs & derivatives
Random Allocation
Rats
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Trifolium chemistry
Estrogen Receptor alpha metabolism
Estrogen Receptor beta metabolism
Heart Atria metabolism
Isoflavones pharmacology
Phytoestrogens pharmacology
Uterus drug effects
Subjects
Details
- Language :
- Chinese
- ISSN :
- 1001-4454
- Volume :
- 33
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
- Publication Type :
- Academic Journal
- Accession number :
- 21243777