[Study on the chemical constituents from fresh roots of Euphorbia fischeriana].

Objective: To study the chemical constituents in ethyl acetate fraction from the Euphorbia fischeriana.
Methods: The compounds were isolated by silica gel column chromatography and HPLC, and their structures were elucidated by means of spectral analyses.
Results: Fourteen compounds were identified as 2,4-dihydroxy-6-methoxy-3-methylacetophenone (1), 12-deoxyphorbol-13-hexadecanoate (2), ethylgallate (3), esculetin (4), 2,4-dihydroxy-6-methoxy-acetophenone (5), 12-deoxyphorbol-13-acetate (6), 12-deoxyphorbol-13,20-diacetete (7), jolkinolide B (8), 17-hydroxyjolkinolide B (9), 17-hydroxy-jolkinolide A (10), langduin C (11), 3-methylp-hydroxybenzoic acid (12), methyl gallate (13), 3, 3'-diacetyl-pyridine-4,4'-dimethoxy-2, 2', 6, 6'-tetrahydroxydiphenylmethane (14).
Conclusion: Compounds 3, 5, 12, 13 are isolated from this plant for the first time.