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Transcriptional and antagonistic responses of Pseudomonas fluorescens Pf0-1 to phylogenetically different bacterial competitors.
- Source :
-
The ISME journal [ISME J] 2011 Jun; Vol. 5 (6), pp. 973-85. Date of Electronic Publication: 2011 Jan 13. - Publication Year :
- 2011
-
Abstract
- The ability of soil bacteria to successfully compete with a range of other microbial species is crucial for their growth and survival in the nutrient-limited soil environment. In the present work, we studied the behavior and transcriptional responses of soil-inhabiting Pseudomonas fluorescens strain Pf0-1 on nutrient-poor agar to confrontation with strains of three phylogenetically different bacterial genera, that is, Bacillus, Brevundimonas and Pedobacter. Competition for nutrients was apparent as all three bacterial genera had a negative effect on the density of P. fluorescens Pf0-1; this effect was most strong during the interaction with Bacillus. Microarray-based analyses indicated strong differences in the transcriptional responses of Pf0-1 to the different competitors. There was higher similarity in the gene expression response of P. fluorescens Pf0-1 to the Gram-negative bacteria as compared with the Gram-positive strain. The Gram-negative strains did also trigger the production of an unknown broad-spectrum antibiotic in Pf0-1. More detailed analysis indicated that expression of specific Pf0-1 genes involved in signal transduction and secondary metabolite production was strongly affected by the competitors' identity, suggesting that Pf0-1 can distinguish among different competitors and fine-tune its competitive strategies. The results presented here demonstrate that P. fluorescens Pf0-1 shows a species-specific transcriptional and metabolic response to bacterial competitors and provide new leads in the identification of specific cues in bacteria-bacteria interactions and of novel competitive strategies, antimicrobial traits and genes.
- Subjects :
- Alphaproteobacteria growth & development
Amino Acids metabolism
Anti-Bacterial Agents biosynthesis
Bacillus growth & development
Bacterial Proteins genetics
Bacteroidetes growth & development
Gene Expression Regulation, Bacterial
Pseudomonas fluorescens genetics
Ribosomal Proteins genetics
Signal Transduction
Transcription, Genetic
Antibiosis
Pseudomonas fluorescens growth & development
Pseudomonas fluorescens metabolism
Soil Microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1751-7370
- Volume :
- 5
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The ISME journal
- Publication Type :
- Academic Journal
- Accession number :
- 21228890
- Full Text :
- https://doi.org/10.1038/ismej.2010.196