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Analysis of cloned structural and regulatory genes for carbohydrate utilization in Pseudomonas aeruginosa PAO.
- Source :
-
Journal of bacteriology [J Bacteriol] 1990 Nov; Vol. 172 (11), pp. 6396-402. - Publication Year :
- 1990
-
Abstract
- Five of the genes required for phosphorylative catabolism of glucose in Pseudomonas aeruginosa were ordered on two different chromosomal fragments. Analysis of a previously isolated 6.0-kb EcoRI fragment containing three structural genes showed that the genes were present on a 4.6-kb fragment in the order glucose-binding protein (gltB)-glucokinase (glk)-6-phosphogluconate dehydratase (edd). Two genes, glucose-6-phosphate dehydrogenase (zwf) and 2-keto-3-deoxy-6-phosphogluconate aldolase (eda), shown by transductional analysis to be linked to gltB and edd, were cloned on a separate 11-kb BamHI chromosomal DNA fragment and then subcloned and ordered on a 7-kb fragment. The 6.0-kb EcoRI fragment had been shown to complement a regulatory mutation, hexR, which caused noninducibility of four glucose catabolic enzymes. In this study, hexR was mapped coincident with edd. A second regulatory function, hexC, was cloned within a 0.6-kb fragment contiguous to the edd gene but containing none of the structural genes. The phenotypic effect of the hexC locus, when present on a multicopy plasmid, was elevated expression of glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydratase, and 2-keto-3-deoxy-6-phosphogluconate aldolase activities in the absence of inducer.
- Subjects :
- Chimera
Chromosomes, Bacterial
DNA, Bacterial genetics
DNA, Bacterial isolation & purification
Genotype
Models, Biological
Plasmids
Pseudomonas aeruginosa metabolism
Restriction Mapping
Carbohydrate Metabolism
Genes, Bacterial
Genes, Regulator
Glucose metabolism
Pseudomonas aeruginosa genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9193
- Volume :
- 172
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of bacteriology
- Publication Type :
- Academic Journal
- Accession number :
- 2121713
- Full Text :
- https://doi.org/10.1128/jb.172.11.6396-6402.1990