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Amniotic fluid interleukin-1 beta and interleukin-6, but not interleukin-8 correlate with microbial invasion of the amniotic cavity in preterm labor.
- Source :
-
American journal of reproductive immunology (New York, N.Y. : 1989) [Am J Reprod Immunol] 2011 Jun; Vol. 65 (6), pp. 549-56. Date of Electronic Publication: 2011 Jan 09. - Publication Year :
- 2011
-
Abstract
- Problem: We compared the frequency of intra-amniotic infection in preterm labor (PL) with women not in labor, and correlated infection with amniotic fluid (AF) cytokines. Detailed identification of species, especially mycoplasmata, was tried to improve our understanding of the pathogenesis of PL.<br />Method of Study: AF from 20 women with PL and 20 controls were evaluated. Infection was detected by PCR for Mycoplasma hominis, Ureaplasma urealyticum and 16S rRNA bacterial gene, which was cloned and sequenced for bacterial identification. Interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α levels were measured by ELISA.<br />Results: Frequency of intra-amniotic infection is higher in PL (40.0%). Sequencing-based method identified Bacteroides fragilis, Prevotella bivia and Leptotrichia amnionii, in addition to Mycoplasma species detected by PCR. AF infection correlated with increased IL-1β and IL-6 levels.<br />Conclusion: The frequency of intra-amniotic infection, especially M. hominis, in PL women who delivered with 7 days, is high and correlates with high IL-1β and IL-6 levels, but not IL-8.<br /> (© 2011 John Wiley & Sons A/S.)
- Subjects :
- Amniotic Fluid immunology
Amniotic Fluid microbiology
Female
Gram-Negative Anaerobic Bacteria pathogenicity
Gram-Negative Bacterial Infections epidemiology
Humans
Interleukin-1beta genetics
Interleukin-1beta immunology
Interleukin-6 genetics
Interleukin-6 immunology
Interleukin-8 genetics
Interleukin-8 immunology
Interleukin-8 metabolism
Mycoplasma Infections epidemiology
Mycoplasma hominis pathogenicity
Obstetric Labor, Premature epidemiology
Obstetric Labor, Premature microbiology
Pregnancy
Prevalence
RNA, Ribosomal, 16S analysis
Sequence Analysis, RNA
Tumor Necrosis Factor-alpha genetics
Tumor Necrosis Factor-alpha immunology
Tumor Necrosis Factor-alpha metabolism
Ureaplasma Infections epidemiology
Ureaplasma urealyticum pathogenicity
Amniotic Fluid metabolism
Gram-Negative Anaerobic Bacteria physiology
Gram-Negative Bacterial Infections immunology
Interleukin-1beta metabolism
Interleukin-6 metabolism
Mycoplasma Infections immunology
Mycoplasma hominis physiology
Obstetric Labor, Premature immunology
Ureaplasma Infections immunology
Ureaplasma urealyticum physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0897
- Volume :
- 65
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- American journal of reproductive immunology (New York, N.Y. : 1989)
- Publication Type :
- Academic Journal
- Accession number :
- 21214658
- Full Text :
- https://doi.org/10.1111/j.1600-0897.2010.00940.x