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In vivo-derived horse blastocysts show transcriptional upregulation of developmentally important genes compared with in vitro-produced horse blastocysts.
- Source :
-
Reproduction, fertility, and development [Reprod Fertil Dev] 2011; Vol. 23 (2), pp. 364-75. - Publication Year :
- 2011
-
Abstract
- In vitro-produced (IVP) equine blastocysts can give rise to successful pregnancies, but their morphology and developmental rate differ from those of in vivo-derived equine blastocysts. The aim of the present study was to evaluate this difference at the genetic level. Suppression subtractive hybridisation (SSH) was used to construct a cDNA library enriched for transcripts preferentially expressed in in vivo-derived equine blastocysts compared with IVP blastocysts. Of the 62 different genes identified in this way, six genes involved in embryonic development (BEX2, FABP3, HSP90AA1, MOBKL3, MCM7 and ODC) were selected to confirm this differential expression by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Using RT-qPCR, five genes were confirmed to be significantly upregulated in in vivo-derived blastocysts (i.e. FABP3, HSP90AA1 (both P<0.05), ODC, MOBKL3 and BEX2 (P<0.005 for all three)), confirming the results of the SSH. There was no significant difference in MCM7 expression between IVP and in vivo-derived blastocysts. In conclusion, five genes that are transcriptionally upregulated in in vivo-derived equine blastocysts compared with IVP blastocysts have been identified. Because of their possible importance in embryonic development, the expression of these genes can be used as a marker to evaluate in vitro embryo production systems in the horse.
- Subjects :
- Adaptor Proteins, Signal Transducing genetics
Animals
Fatty Acid-Binding Proteins genetics
Female
HSP90 Heat-Shock Proteins genetics
Nerve Tissue Proteins genetics
Nucleic Acid Hybridization
Ornithine Decarboxylase genetics
Pregnancy
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Blastocyst metabolism
Fertilization in Vitro veterinary
Gene Expression Regulation, Developmental
Horses embryology
Subjects
Details
- Language :
- English
- ISSN :
- 1031-3613
- Volume :
- 23
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Reproduction, fertility, and development
- Publication Type :
- Academic Journal
- Accession number :
- 21211470
- Full Text :
- https://doi.org/10.1071/RD10124