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Inflammatory lipid mediator generation elicited by viable hemolysin-forming Escherichia coli in lung vasculature.
- Source :
-
The Journal of experimental medicine [J Exp Med] 1990 Oct 01; Vol. 172 (4), pp. 1115-25. - Publication Year :
- 1990
-
Abstract
- Escherichia coli hemolysin, a transmembrane pore-forming exotoxin, is considered an important virulence factor for E. coli-related extraintestinal infections and sepsis. The possible significance of hemolysin liberation for induction of inflammatory lipid mediators was investigated in isolated rabbit lungs infused with viable bacteria (concentration range, 10(4)-10(7)/ml). Hemolysin-secreting E. coli (E. coli-Hly+), but not an E. coli strain that releases an inactive form of the exotoxin, induced marked lung leukotriene (LT) generation with predominance of cysteinyl LTs. Eicosanoid synthesis was not inhibited in the presence of plasma with toxin-neutralizing capacity. Pre-application of 2 x 10(8) human granulocytes, which sequestered in the lung microvasculature, caused a severalfold increase in leukotriene generation in response to E. coli-Hly+ challenge both in the absence and presence of plasma. Data are presented indicating neutrophil-endothelial cell cooperation in arachidonic acid lipoxygenase metabolism as an underlying mechanism. We conclude that liberation of hemolysin from viable E. coli induces marked lipid mediator generation in lung vasculature, which is potentiated in the presence of neutrophil sequestration and may contribute to microcirculatory disturbances during the course of severe infections.
- Subjects :
- Animals
Arachidonic Acid
Arachidonic Acids metabolism
Female
Humans
Hydroxyeicosatetraenoic Acids metabolism
Lung blood supply
Male
Microcirculation metabolism
Neutrophils physiology
Perfusion
Rabbits
Escherichia coli pathogenicity
Hemolysin Proteins toxicity
Leukotrienes metabolism
Lung metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1007
- Volume :
- 172
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of experimental medicine
- Publication Type :
- Academic Journal
- Accession number :
- 2120384
- Full Text :
- https://doi.org/10.1084/jem.172.4.1115