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Molecular analysis of a germ line-encoded idiotypic marker of pathogenic human lupus autoantibodies.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1990 Oct 15; Vol. 145 (8), pp. 2545-53. - Publication Year :
- 1990
-
Abstract
- Id-16/6 is an idiotypic marker found in both IgM and IgG antibodies, as well as in the tissue lesions of patients with SLE. The prototypic Id-16/6+ mAb is 18/2, whose VH3-derived H chain is encoded by an unmutated germ-line gene. We found that the H chains of VH3-derived Id-16/6+ antibodies contain the major determinants of Id-16/6. Moreover, B cell clones from which those antibodies were harvested produce RNA that hybridized under conditions of high stringency to oligonucleotide probes corresponding to the CDR of the VH segment of 18/2. Western blots of Id-16/6+ mAbs with anti-Id confirmed the association of the Id with H chains. Id-16/6 can identify a subgroup of VH3-derived antibodies we have termed the 18/2 CDR family. However, Id-16/6 can also be expressed in some antibodies unrelated to the 18/2 CDR family. No characteristic Ag-binding specificity was found among the members of the 18/2 CDR family. The principal phenotypic feature shared by all known members of the family is Id-16/6.
- Subjects :
- Amino Acid Sequence
Antibodies, Monoclonal
Antibody Specificity
Autoantibodies genetics
B-Lymphocytes immunology
Blotting, Western
Genes, Immunoglobulin
Humans
Immunoglobulin Heavy Chains genetics
Immunoglobulin Heavy Chains immunology
Immunoglobulin Idiotypes genetics
Immunoglobulin M genetics
Immunoglobulin M immunology
Immunoglobulin Variable Region genetics
Immunoglobulin Variable Region immunology
Molecular Sequence Data
Autoantibodies immunology
Immunoglobulin Idiotypes immunology
Lupus Erythematosus, Systemic immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 145
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 2120334