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Expression profiling of Ca(2+)/calmodulin-dependent signaling molecules in the rat dorsal and ventral hippocampus after acute lead exposure.
- Source :
-
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie [Exp Toxicol Pathol] 2012 Sep; Vol. 64 (6), pp. 619-24. Date of Electronic Publication: 2010 Dec 30. - Publication Year :
- 2012
-
Abstract
- The septal and temporal poles of the hippocampus differ markedly in their anatomical organization, but whether these distinct regions exhibit differential neurochemical profiles underlying lead (Pb(2+)) neurotoxicity remains to be determined. In the present study, we examined changes in the expression of Ca(2+)/calmodulin-dependent enzymes, including calpain, calcineurin, phospho-CaMKII (Thr286) and neuronal nitric oxide synthase (nNOS), in the rat dorsal and ventral hippocampus (DH and VH) after acute Pb(2+) exposure. Five days after Pb(2+) exposure, we observed constitutively active forms of calcineurin (45 kDa and 48 kDa) in ventral portions of the hippocampus, a result consistent with the observed calpain activation that is indicated by the breakdown of spectrin in this region. Our data demonstrate that nNOS expression is significantly higher in the ventral region of the hippocampus when compared to the dorsal region, whereas phosphorylation of CaMKII (Thr286) is less pronounced in the ventral portion of the hippocampus and more pronounced in dorsal regions after acute Pb(2+) exposure. Thus, it appears likely that the ventral region of hippocampus is more vulnerable to the neurotoxic effects of Pb(2+) than the dorsal region. Taken together, the present data suggest that acute lead exposure leads to differential expression patterns of Ca(2+)/calmodulin-dependent enzymes along the dorsoventral axis of the hippocampus.<br /> (Copyright © 2011 Elsevier GmbH. All rights reserved.)
- Subjects :
- Animals
Blotting, Western
Calcium-Calmodulin-Dependent Protein Kinase Type 2 analysis
Calpain analysis
Hippocampus metabolism
Immunohistochemistry
Male
Nitric Oxide Synthase Type I analysis
Rats
Rats, Sprague-Dawley
Calcium Signaling drug effects
Calcium-Calmodulin-Dependent Protein Kinase Type 2 metabolism
Calpain metabolism
Hippocampus drug effects
Lead toxicity
Nitric Oxide Synthase Type I metabolism
Transcriptome drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1618-1433
- Volume :
- 64
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie
- Publication Type :
- Academic Journal
- Accession number :
- 21195594
- Full Text :
- https://doi.org/10.1016/j.etp.2010.12.004