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Drug permeation across intestinal epithelial cells using porous silicon nanoparticles.
- Source :
-
Biomaterials [Biomaterials] 2011 Apr; Vol. 32 (10), pp. 2625-33. Date of Electronic Publication: 2010 Dec 30. - Publication Year :
- 2011
-
Abstract
- Mesoporous silicon particles hold great potential in improving the solubility of otherwise poorly soluble drugs. To effectively translate this feature into the clinic, especially via oral or parenteral administration, a thorough understanding of the interactions of the micro- and nanosized material with the physiological environment during the delivery process is required. In the present study, the behaviour of thermally oxidized porous silicon particles of different sizes interacting with Caco-2 cells (both non-differentiated and polarized monolayers) was investigated in order to establish their fate in a model of intestinal epithelial cell barrier. Particle interactions and TNF-α were measured in RAW 264.7 macrophages, while cell viabilities, reactive oxygen species and nitric oxide levels, together with transmission electron microscope images of the polarized monolayers, were assessed with both the Caco-2 cells and RAW 264.7 macrophages. The results showed a concentration and size dependent influence on cell viability and ROS-, NO- and TNF-α levels. There was no evidence of the porous nanoparticles crossing the Caco-2 cell monolayers, yet increased permeation of the loaded poorly soluble drug, griseofulvin, was shown.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Caco-2 Cells
Cell Death drug effects
Cell Survival drug effects
Epithelial Cells pathology
Epithelial Cells ultrastructure
Humans
Inflammation pathology
Intracellular Space drug effects
Intracellular Space metabolism
Macrophages drug effects
Macrophages metabolism
Macrophages pathology
Mice
Nanoparticles ultrastructure
Nitric Oxide biosynthesis
Oxidation-Reduction drug effects
Porosity drug effects
Reactive Oxygen Species metabolism
Solubility drug effects
Temperature
Tumor Necrosis Factor-alpha metabolism
Cell Membrane Permeability drug effects
Epithelial Cells drug effects
Epithelial Cells metabolism
Griseofulvin pharmacology
Intestines cytology
Nanoparticles chemistry
Silicon pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 32
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 21194747
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.12.011