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Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death.

Authors :
Richard E
Causse S
Spriet C
Fourré N
Trinel D
Darzacq X
Vandenbunder B
Heliot L
Source :
Photochemistry and photobiology [Photochem Photobiol] 2011 Jan-Feb; Vol. 87 (1), pp. 256-61. Date of Electronic Publication: 2010 Dec 10.
Publication Year :
2011

Abstract

The fluorescent probe DRAQ5 which rapidly permeates cells and binds to DNA is potentially useful for functional studies of molecular dynamics and interactions in living nuclei. Within minutes after the incubation of human osteosarcoma U2OS cells with 5μm DRAQ5, the distributions of RNA polymerase II and some of its associated regulatory proteins HEXIM and cyclin T1 in the nucleus are severely impaired, and transcription is inhibited. Furthermore, 30min exposure to DRAQ5 induces death of U2OS cells 24h later. Incubation with Hoechst 33342 under similar conditions does not induce these effects. These results emphasize the importance of carefully examining the functional consequences of labeling DNA with intercalating fluorescent dyes before use.<br /> (© 2010 The Authors. Photochemistry and Photobiology © 2010 The American Society of Photobiology.)

Details

Language :
English
ISSN :
1751-1097
Volume :
87
Issue :
1
Database :
MEDLINE
Journal :
Photochemistry and photobiology
Publication Type :
Academic Journal
Accession number :
21175643
Full Text :
https://doi.org/10.1111/j.1751-1097.2010.00852.x