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Processing of fish Ig heavy chain transcripts: diverse splicing patterns and unusual nonsense mediated decay.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2011 Sep; Vol. 35 (9), pp. 949-58. Date of Electronic Publication: 2010 Dec 17. - Publication Year :
- 2011
-
Abstract
- While the diversification of the antigen-binding sites is realized by genomic VDJ rearrangements during B cell differentiation, different forms of immunoglobulin (Ig) heavy (H) chains can be produced through multiple splicing pathways. In most vertebrates, the secreted (S) and membrane (Mb) forms of IgM chain are created by alternative splicing through usage of a cryptic splice site in Cμ4 allowing the junction to the TM exon. The processing pattern for Igμ is different in teleosts, which generally use the Cμ3 donor site instead. In ancient fish lineages, multiple unusual splicing patterns were found for Ig H chain, involving donor sites that do not always follow the classical consensus. The production of IgD versus IgM H chains seems to be generally realized by alternative splicing in all vertebrates, but typical teleost IgD H chains are chimeric and contains a Cμ1 domain. Together, these observations raise questions on how different fish regulate RNA splicing and if their splicing machinery is especially complex. A preliminary scan of the zebrafish and stickleback genomes provides evidence that gene orthologs to the mammalian main splice factors are highly conserved as single copy genes, while the snRNPs U repertoire may be different and may explain other particular features of RNA processing in fish.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 35
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 21168434
- Full Text :
- https://doi.org/10.1016/j.dci.2010.12.007