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Identification of a luxO-regulated extracellular protein Pep and its roles in motility in Vibrio alginolyticus.
- Source :
-
Microbial pathogenesis [Microb Pathog] 2011 Feb; Vol. 50 (2), pp. 123-31. Date of Electronic Publication: 2010 Dec 15. - Publication Year :
- 2011
-
Abstract
- The fish pathogen Vibrio alginolyticus virulence factors and regulation mechanism are barely known except the quorum sensing regulated extracellular products. In the present study, the cleavable isotope-coded affinity tags (cICATs) were used to analyze the differentially expressed extracellular proteins of V. alginolyticus wild-type and a ΔluxO mutant, and a putative peptidase Pep was identified. Quantitative real-time reverse transcription PCR confirmed that the transcription of pep was down-regulated by LuxO. Furthermore, western blotting demonstrated that the expression of Pep was cell-density dependent and quorum sensing regulated in V. alginolyticus. The pep in-frame mutant abolished the swimming and swarming motility, suggesting that it was involved in motility regulation in V. alginolyticus. In liquid medium, the polar flagellum was observed intact in ∆pep mutant while the chemotaxis related gene cheY, which is a response regulator causing change in direction of flagellar rotation, was down-regulated in ∆pep mutant. When ∆pep mutant was grown on hard agar plate, the lateral flagella disappeared and the transcriptions of flagellar biogenesis genes flaK, fliS, lafK, and lafA were down-regulated in ∆pep mutant. These results suggested that Pep was a luxO-regulated extracellular protein and involved in the motility of V. alginolyticus.<br /> (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Bacterial Proteins genetics
Flagella genetics
Flagella metabolism
Molecular Sequence Data
Peptide Hydrolases genetics
Quorum Sensing
Repressor Proteins genetics
Vibrio alginolyticus enzymology
Vibrio alginolyticus genetics
Bacterial Proteins metabolism
Gene Expression Regulation, Bacterial
Peptide Hydrolases metabolism
Repressor Proteins metabolism
Vibrio alginolyticus physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1208
- Volume :
- 50
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Microbial pathogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 21167274
- Full Text :
- https://doi.org/10.1016/j.micpath.2010.12.003