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Molecular imaging techniques to study the biodistribution of orally administered (99m)Tc-labelled naive and ligand-tagged nanoparticles.
- Source :
-
Molecular imaging and biology [Mol Imaging Biol] 2011 Dec; Vol. 13 (6), pp. 1215-23. - Publication Year :
- 2011
-
Abstract
- Purpose: Study by molecular imaging the biodistribution of poly(anhydride) nanoparticles after oral administration.<br />Procedures: Poly (anhydride) nanoparticles (NP) and cyclodextrin-tagged nanoparticles (CD-NP) were radiolabelled with (99m)Tc. Radiochemical purity was measured with a double-solvent chromatography system and the absence of undesirable components was confirmed by size and polydispersion measurement of the technetium-labelled nanoparticles by photon correlation spectroscopy. Single photon emission computed tomography (SPECT) fused computed tomography (CT) in vivo molecular imaging was used for biodistribution studies in small animals.<br />Results: SPECT-CT images revealed activity only in the gastrointestinal tract. Thirteen percent of the given dose of CD-NP and 3% of the given dose of conventional NP were found in the stomach at 8 h.<br />Conclusion: No evidence of translocation or distribution out of gastrointestinal tract was found. CD-NP moved significantly more slowly inside the gut than conventional NP, probably due to their physico-chemical structure that allows stronger interactions with the gut mucosa.
- Subjects :
- Administration, Oral
Animals
Cyclodextrins chemistry
Freeze Drying
Gastric Emptying
Ligands
Male
Nanoparticles ultrastructure
Quality Control
Rats
Rats, Wistar
Spectrum Analysis
Tissue Distribution
Tomography, Emission-Computed, Single-Photon
Tomography, X-Ray Computed
Molecular Imaging methods
Nanoparticles chemistry
Technetium administration & dosage
Technetium pharmacokinetics
Subjects
Details
- Language :
- English
- ISSN :
- 1860-2002
- Volume :
- 13
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Molecular imaging and biology
- Publication Type :
- Academic Journal
- Accession number :
- 21161691
- Full Text :
- https://doi.org/10.1007/s11307-010-0456-0