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Glutamate induces glutathione efflux mediated by glutamate/aspartate transporter in retinal cell cultures.
- Source :
-
Neurochemical research [Neurochem Res] 2011 Mar; Vol. 36 (3), pp. 412-8. Date of Electronic Publication: 2010 Dec 16. - Publication Year :
- 2011
-
Abstract
- This study was undertaken in order to characterize the role of the glutamate/aspartate transporter (GLAST) in the glutathione (GSH) efflux induced by glutamate. Our results demonstrated that retinal cell cultures exhibit two mechanisms of GSH release, one Na(+)-independent and other Na(+)-dependent. Glutamate and aspartate induced GSH efflux only in presence of Na(+). Treatment with PCD (L-trans-Pyrrolidine-2,4-dicarboxylate), a transportable glutamate uptake blocker, increased GSH release indicating that GSH can be carried by glutamate transporters in retinal cell cultures. Added to this, treatment with zinc ion cultures, a recognized inhibitor of GLAST blocked GSH efflux evoked by glutamate. Treatment with NMDA antagonist (MK-801) did not have any effect on the GSH release induced by glutamate. These results suggest that glutamate induces GLAST-mediated release of GSH from retinal cell cultures and this could represent an important mechanism of cellular protection against glutamate toxicity in the CNS.
- Subjects :
- Animals
Aspartic Acid pharmacology
Cells, Cultured
Chick Embryo
Dicarboxylic Acids pharmacology
Glutamic Acid metabolism
Neurotransmitter Uptake Inhibitors pharmacology
Pyrrolidines pharmacology
Retina drug effects
Amino Acid Transport System X-AG metabolism
Glutamic Acid pharmacology
Glutathione metabolism
Retina cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1573-6903
- Volume :
- 36
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neurochemical research
- Publication Type :
- Academic Journal
- Accession number :
- 21161593
- Full Text :
- https://doi.org/10.1007/s11064-010-0356-3