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Effect of probiotic-fermented milk administration on gastrointestinal survival of Lactobacillus casei ATCC 393 and modulation of intestinal microbial flora.
- Source :
-
Journal of molecular microbiology and biotechnology [J Mol Microbiol Biotechnol] 2010; Vol. 19 (4), pp. 224-30. Date of Electronic Publication: 2010 Dec 14. - Publication Year :
- 2010
-
Abstract
- The aim of the present study was to assess the survival of free and immobilized Lactobacillus casei ATCC 393 on apple pieces, contained in probiotic-fermented milk, after gastrointestinal (GI) transit and to investigate the potential regulation of intestinal microbial flora in a rat model. In in vitro GI stress tolerance tests, immobilized L. casei ATCC 393 exhibited significantly higher survival rates compared to free cells. At a second stage, probiotic-fermented milk produced by either free or immobilized cells was administered orally at a single dose or daily for 9 days in Wistar rats. By 12 h after single-dose administration, both free and immobilized cells were detected by microbiological and molecular analysis at levels ≥6 logCFU/g of feces. Moreover, daily administration led to significant reduction of staphylococci, enterobacteria, coliforms and streptococci counts. In conclusion, L. casei ATCC 393 contained in fermented milk survived GI transit and modulated intestinal microbiota.<br /> (Copyright © 2010 S. Karger AG, Basel.)
- Subjects :
- Animals
Bile Acids and Salts pharmacology
Gastric Juice microbiology
Lacticaseibacillus casei growth & development
Lacticaseibacillus casei metabolism
Malus microbiology
Rats
Cultured Milk Products microbiology
Gastrointestinal Tract microbiology
Lacticaseibacillus casei physiology
Metagenome physiology
Microbial Viability drug effects
Probiotics
Subjects
Details
- Language :
- English
- ISSN :
- 1660-2412
- Volume :
- 19
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of molecular microbiology and biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 21160205
- Full Text :
- https://doi.org/10.1159/000321115